File(s) under permanent embargo
Maternal dietary creatine supplementation does not alter the capacity for creatine synthesis in the newborn spiny mouse
journal contribution
posted on 2013-09-01, 00:00 authored by H Dickinson, Z Ireland, D La Rosa, Bree Aimee O'Connell, S Ellery, Rod SnowRod Snow, D WalkerWe have previously reported that maternal creatine supplementation protects the neonate from hypoxic injury. Here, we investigated whether maternal creatine supplementation altered expression of the creatine synthesis enzymes (arginine:glycine amidinotransferase [AGAT], guanidinoaceteate methyltransferase [GAMT]) and the creatine transporter (solute carrier family 6 [neurotransmitter transporter, creatine] member 8: SLC6A8) in the term offspring. Pregnant spiny mice were fed a 5% creatine monohydrate diet from midgestation (day 20) to term (39 days). Placentas and neonatal kidney, liver, heart, and brain collected at 24 hours of age underwent quantitative polymerase chain reaction and Western blot analysis. Maternal creatine had no effect on the expression of AGAT and GAMT in neonatal kidney and liver, but mRNA expression of AGAT in brain tissues was significantly decreased in both male and female neonates born to mothers who were fed the creatine diet. SLC6A8 expression was not affected by maternal dietary creatine loading in any tissues. Maternal dietary creatine supplementation from midgestation in the spiny mouse did not alter the capacity for creatine synthesis or transport.
History
Journal
Reproductive sciencesVolume
20Issue
9Pagination
1096 - 1102Publisher
Sage PublicationsLocation
Thousand Oaks, CaliforniaPublisher DOI
ISSN
1933-7191eISSN
1933-7205Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2013, Sage PublishingUsage metrics
Categories
No categories selectedKeywords
maternal creatine supplementationcreatine synthesis enzymescreatine transporterScience & TechnologyLife Sciences & BiomedicineObstetrics & GynecologyReproductive BiologySLC6A8arginine:glycine amidinotransferase (AGAT)guanidinoaceteate methyltransferase (GAMT)neonateplacentaMID-PREGNANCY PROTECTSTRANSPORTEREXPRESSION
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC