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Synthesis, characterisation and preliminary analytical evaluation of three oxamide reagents for peroxyoxalate chemiluminescence
journal contribution
posted on 2000-01-03, 00:00 authored by Neil BarnettNeil Barnett, R Bos, R N Evans, R A RussellThe synthesis, characterisation and preliminary analytical evaluation of three oxamide reagents, for peroxyoxalate chemiluminescence, are described. The analytical figures of merit for the three oxamides were assessed using flow injection analysis and the fluorophores rhodamine B and fluorescein in a solvent mixture consisting of tetrahydrofuran, acetonitrile and aqueous buffer (1+1+2 by volume). The calibration functions approached linearity and the best detection limit (for both analytes) was 2 x 10-9M when using the oxamide 2,2'-oxalyl-bis[(trifluoromethanesulfonyl)imino]ethylene-bis(N- methylpyridinium)trifluoromethanesulfonate. These results were found to compare favourably with those obtained for, the popular peroxyoxalate chemiluminescence reagent, bis(2,4,6-trichlorophenyl) oxalate (TCPO), using an identical solvent system. However, the detection limits achieved with TCPO were degraded at pH 10 due to a significant increase in the background emission. The addition of the base catalyst, imidazole, to these chemiluminescent reactions resulted in the deterioration of analytical performance for both TCPO and the oxamide reagents.
History
Journal
Analytica chimica actaVolume
403Issue
1-2Pagination
145 - 154Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0003-2670Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2000, Elsevier Science B.V.Usage metrics
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Peroxyoxalate chemiluminescenceSynthesis of oxamidesBis(2,4,6-trichlorophenyl) oxalateFlow injection analysisScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryCHEMI-LUMINESCENCE DETECTIONHYDROGEN-PEROXIDELIQUID-CHROMATOGRAPHYBACKGROUND EMISSIONBIS(2,4-DINITROPHENYL) OXALATEIMIDAZOLE1,1'-OXALYLDIIMIDAZOLEINTERMEDIATEMECHANISM
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