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Multiple Niche Compartments Orchestrate Stepwise Germline Stem Cell Progeny Differentiation
journal contribution
posted on 2023-11-14, 04:10 authored by Renjun Tu, Bo Duan, Xiaoqing Song, Shiyuan Chen, Allison Scott, Kate HallKate Hall, Jillian Blanck, Dustin DeGraffenreid, Hua Li, Anoja Perera, Jeff Haug, Ting XieThe niche controls stem cell self-renewal and progenitor differentiation for maintaining adult tissue homeostasis in various organisms. However, it remains unclear whether the niche is compartmentalized to control stem cell self-renewal and stepwise progeny differentiation. In the Drosophila ovary, inner germarial sheath (IGS) cells form a niche for controlling germline stem cell (GSC) progeny differentiation. In this study, we have identified four IGS subpopulations, which form linearly arranged niche compartments for controlling GSC maintenance and multi-step progeny differentiation. Single-cell analysis of the adult ovary has identified four IGS subpopulations (IGS1-IGS4), the identities and cellular locations of which have been further confirmed by fluorescent in situ hybridization. IGS1 and IGS2 physically interact with GSCs and mitotic cysts to control GSC maintenance and cyst formation, respectively, whereas IGS3 and IGS4 physically interact with 16-cell cysts to regulate meiosis, oocyte development, and cyst morphological change. Finally, one follicle cell progenitor population has also been transcriptionally defined for facilitating future studies on follicle stem cell regulation. Therefore, this study has structurally revealed that the niche is organized into multiple compartments for orchestrating stepwise adult stem cell development and has also provided useful resources and tools for further functional characterization of the niche in the future.
History
Journal
CURRENT BIOLOGYVolume
31Pagination
827-+Location
EnglandPublisher DOI
ISSN
0960-9822eISSN
1879-0445Language
EnglishPublication classification
C1.1 Refereed article in a scholarly journalIssue
4Publisher
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Keywords
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiologyCell BiologyLife Sciences & Biomedicine - Other TopicsDrosophiladifferentiation nicheegg chamberfollicle cell progenitorgermline cyst formationgermline stem celloocyte determinationovary developmentsingle-cell analysisAnimalsCell DifferentiationCystsDrosophila ProteinsFemaleGerm CellsIn Situ Hybridization, FluorescenceStem Cell NicheStem CellsContraception/ReproductionRegenerative MedicineStem Cell Research - Nonembryonic - Non-HumanStem Cell Research1 Underpinning research1.1 Normal biological development and functioningGeneric health relevance
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