Modulation of autoimmune arthritis severity in mice by Apolipoprotein E (ApoE) and cholesterol
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URI: http://hdl.handle.net/10902/10916DOI: 10.1111/cei.12857
ISSN: 0009-9104
ISSN: 1365-2249
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Álvarez Sainz de la Maza, Pilar; Genre, Fernanda; Iglesias Lozano, Marcos; Augustín Rodríguez, Juan Jesús; Tamayo Revuelta, Esther; Escolá Gil, Joan Carles; Blanco Vaca, Francisco; Merino Pérez, Ramón; Merino Pérez, JesúsFecha
2016Derechos
© Wiley. This is the peer reviewed version of the following article: Alvarez, P., Genre, F., Iglesias, M., Augustin, J. J., Tamayo, E., Escolà-Gil, J. C., Lavín, B., Blanco-Vaca, F., Merino, R. and Merino, J. (2016), Modulation of autoimmune arthritis severity in mice by apolipoprotein E (ApoE) and cholesterol. Clin Exp Immunol, 186: 292–303, which has been published in final form at doi:10.1111/cei.12857. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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Clin Exp Immunol. 2016 Aug 29
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Wiley-Blackwell
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Resumen/Abstract
Apolipoprotein E (ApoE) deficiency promoted an exacerbation of autoimmune arthritis in mice by inducing pro-inflammatory immune responses. In this study we analysed the contribution of hypercholesterolemia and/or the absence of ApoE anti-inflammatory properties, unrelated to its function in the control of cholesterol metabolism, towards the acceleration of arthritis in these mutant animals. The induction and severity of collagen type II-induced arthritis (CIA) were compared for B10.RIII wild type (WT), B10.RIII.ApoE+/- , B10.RIII.ApoE-/- and B10.RIII.LDLR-/- mice with different concentrations of circulating ApoE and cholesterol. A 50-70% reduction in serum levels of ApoE was observed in heterozygous B10.RIII.ApoE+/- mice in comparison to B10.RIII.WT, although both strains of mice exhibited similar circulating lipid profiles. This ApoE reduction was associated with an increased CIA severity that remained lower than in homozygous B10.RIII.ApoE-/- mice. An important rise in circulating ApoE concentration was observed in hypercholesterolemic B10.RIII.LDLR-/- mice fed with a normal chow diet, and both parameters further increased with an atherogenic hypercholesterolemic diet. However the severity of CIA in B10.RIII.LDLR-/- mice was similar to that of B10.RIII.WT controls. In conclusion, by comparing the evolution of CIA between several strains of mutant mice with different levels of serum ApoE and cholesterol, our results demonstrate that both hypercholesterolemia and ApoE regulate the intensity of in vivo systemic autoimmune responses. This article is protected by copyright. All rights reserved
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