Detection of antibodies to denatured human leucocyte antigen molecules by single antigen Luminex
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Identificadores
URI: http://hdl.handle.net/10902/21807DOI: 10.1111/tan.14098
ISSN: 2059-2302
ISSN: 2059-2310
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Gutiérrez Larrañaga, María; Riesco, Laura; Guiral, Sandra; Irure Ventura, Juan; Rodrigo Calabia, Emilio; Ocejo Vinyals, Javier; Martorell, Jaume; Caro, Jose L.; López Hoyos, Marcos; San Segundo, DavidFecha
2020-10-10Derechos
his is the pre-peer reviewed version of the following article: [Gutiérrez-Larrañaga M,Riesco L, Guiral S, et al. Detection of antibodies todenatured human leucocyte antigen molecules bysingle antigen Luminex. HLA. 2021;97:52?59.https://doi.org/10.1111/tan.1 4098], which has been published in final form at [https://onlinelibrary.wiley.com/doi/epdf/10.1111/tan.14098]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
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HLA. 2021;97(1):52-59
Editorial
Wiley Blackwell
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Palabras clave
HLA antigens
Immunology
Antibodies
Flow cytometry
Microspheres
Cytotoxicity Tests
Histocompatibility testing
Resumen/Abstract
The anti-HLA antibody detection has been improved in sensitivity and specificity with solid-phase antigen bead (SAB) assays based on Luminex. However, false positive results due to denatured HLA (dHLA) may arise after single antigen test. The aim of this study was to compare the performance of the two Luminex technology-based anti-HLA detection kits available in the market in showing undesired anti-HLA antibody results. A prospective cohort was assessed for anti-HLA antibodies with single antigen A manufacturer (AM) kit and a comparison cohort with single antigen B manufacturer (BM) kit. A total of 11 out of 90 patients in a prospective cohort presented monospecific HLA-I antibodies with AM, and 5 out of 11 confirmed monospecific reaction with BM. Despite the confirmation of monospecific reaction with both manufacturers, 80% were assigned as dHLA reaction by specific crossmatch. Further comparative cohorts detected four out of six monospecific reactions with BM that were confirmed as possible dHLA reactions. A positive SAB test should rule out a reaction against a dHLA molecule, thus avoidance of prolonged waitlist periods or misattribution of anti-HLA reactions after transplantation.
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