Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification

0559191 - BTÚ 2023 RIV GB eng J - Článek v odborném periodiku
Petitpre, Ch. - Faure, L. - Uhl, P. - Fontanet, P. - Filova, Iva - Pavlínková, Gabriela - Adameyko, I. - Hadjab, S. - Lallemend, F.
Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification.
Nature Communications. Roč. 13, č. 1 (2022), č. článku 3878. E-ISSN 2041-1723
Grant CEP: GA ČR(CZ) GA20-06927S
Institucionální podpora: RVO:86652036
Klíčová slova: SPIRAL GANGLION NEURONS * HAIR-CELLS * DEVELOPMENTAL REGULATION
Obor OECD: Developmental biology
Impakt faktor: 16.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41467-022-31580-1

Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that govern their identity and organization in mice. We identify a core set of temporally patterned genes and gene regulatory networks that may contribute to the diversification of SGNs through sequential binary decisions and demonstrate a role for NEUROD1 in driving specification of a I-c-SGN phenotype. We also find that each trajectory of the decision tree is defined by initial co-expression of alternative subtype molecular controls followed by gradual shifts toward cell fate resolution. Finally, analysis of both developing SGN and HC types reveals cell-cell signaling potentially playing a role in the differentiation of SGNs. Our results indicate that SGN identities are drafted prior to birth and reveal molecular principles that shape their differentiation and will facilitate studies of their development, physiology, and dysfunction.
Trvalý link: https://hdl.handle.net/11104/0332909