Fiber optic probe with antifouling polymer brush biointerface for bi- modal biosensing in complex liquid samples

0559043 - FZÚ 2023 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Hasler, R. - Víšová, Ivana - Vrabcová, Markéta - Houska, Milan - Spasovová, Monika - Vaisocherová-Lísalová, Hana - Dostálek, Jakub
Fiber optic probe with antifouling polymer brush biointerface for bi- modal biosensing in complex liquid samples.
Proceedings of SPIE. Bellingham: SPIE, 2022 - (Canva, M.; Giannetti, A.; Altug, H.; Moreau, J.), č. článku 1214505. 12145. ISSN 0277-786X.
[SPIE Photonics Europe 2022. Strasbourg (FR), 03.04.2022-23.05.2022]
Grant CEP: GA ČR(CZ) GA21-19779S; GA ČR(CZ) GC22-30456J
Grant ostatní: AV ČR(CZ) LQ100101902
Program: Prémie Lumina quaeruntur
Institucionální podpora: RVO:68378271
Klíčová slova: surface plasmon resonance * field effect transistor * cyclic voltammetry * biosensor * antifouling polymer brush
Obor OECD: Biophysics
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12145/2621192/Fiber-optic-probe-with-antifouling-polymer-brush-biointerface-for-bi/10.1117/12.2621192.short?SSO=1

We report on the utilization of an antifouling brush biointerface at the surface of surface plasmon resonance (SPR) optical probe for real-world biosensor applications. This biointerface architecture provides the advantage of resistance to fouling when contacted with complex biological fluids, it can be postmodified with functional biomolecular species for affinity biosensor applications, and allows for combined optical and electronic-based biosensing. The SPR optical probe carrying the thin gold film and antifouling polymer brush architecture is successfully implemented as a gate electrode in electrolyte-gated field effect transistor (EG-FET) readout and as a working electrode in cyclic voltammetry (CV). The poly(carboxybetaine acrylamide) (pCBAA) brush was synthesized by the ‘grafting from’ approach with a thickness matching the evanescent field of surface plasmons.
Trvalý link: https://hdl.handle.net/11104/0333113