Photon-upconversion sensing in droplet microfluidics

0565010 - ÚIACH 2023 CZ eng A - Abstrakt
Křivánková, Jana - Hlaváček, Antonín - Foret, František
Photon-upconversion sensing in droplet microfluidics.
Proceedings of APCE-CECE-ITP-IUPAC 2022. Brno: Ústav analytické chemie AV ČR, v. v. i., 2022 - (Foret, F.; Lavická, J.; Chung, D.). s. 174-174. ISBN 978-80-904959-9-9.
[APCE-CECE-ITP-IUPAC 2022. 06.11.2022-10.11.2022, Angkor Wat]
Grant CEP: GA ČR(CZ) GA21-03156S
Institucionální podpora: RVO:68081715
Klíčová slova: cancer biomarkers * droplet microfluidics * photon-upconversion
Obor OECD: Analytical chemistry
https://hdl.handle.net/11104/0336571

Droplet microfluidics allows the acquisition of large amounts of data with only minute consumption of reagents. Therefore, they present an example of a high-throughput tool for biological and biomedicine applications. The detection of cancer biomarkers plays a significant role in clinical diagnostics. However, the sensitivity of current methods is limited. We are overcoming the limitations of fluorescence labels by introducing photonupconversion nanoparticles for microfluidic assays. These nanoparticles (diameter 1–100 nm) emit short wavelengths under near-infrared excitation (976 nm), which mitigates optical background interferences (autofluorescence, light scattering). This unique photoluminescent feature enables the detection of photonupconversion nanoparticles at the single nanoparticle level by conventional wide-field microscopy. Here we present novel instrumentation for recording photon-upconversion from water microdroplets in affordable polydimethylsiloxane microfluidic chips on a glass substrate.
Trvalý link: https://hdl.handle.net/11104/0336580