Influence of hydrophobic side-chain length in amphiphilic gradient copoly(2-oxazoline)s on the therapeutics loading, stability, cellular uptake and pharmacokinetics of nano-formulation with curcumin

0564413 - ÚMCH 2023 RIV CH eng J - Článek v odborném periodiku
Datta, S. - Huntošová, V. - Jutková, A. - Seliga, R. - Kronek, J. - Tomkova, A. - Lenkavská, L. - Máčajová, M. - Bilčík, B. - Kundeková, B. - Čavarga, I. - Pavlova, Ewa - Šlouf, Miroslav - Miškovský, P. - Jančura, D.
Influence of hydrophobic side-chain length in amphiphilic gradient copoly(2-oxazoline)s on the therapeutics loading, stability, cellular uptake and pharmacokinetics of nano-formulation with curcumin.
Pharmaceutics. Roč. 14, č. 12 (2022), č. článku 2576. E-ISSN 1999-4923
Institucionální podpora: RVO:61389013
Klíčová slova: polymeric nanoparticles * hydrophobicity * curcumin
Obor OECD: Polymer science
Impakt faktor: 5.4, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/1999-4923/14/12/2576

Due to the simple one-step preparation method and a promising application in biomedical research, amphiphilic gradient copoly(2-oxazoline)s are gaining more and more interest compared to their analogous block copolymers. In this work, the curcumin solubilization ability was tested for a series of amphiphilic gradient copoly(2-oxazoline)s with different lengths of hydrophobic side-chains, consisting of 2-ethyl-2-oxazoline as a hydrophilic monomer and 2-(4-alkyloxyphenyl)-2-oxazoline as a hydrophobic monomer. It is shown that the length of the hydrophobic side-chain in the copolymers plays a crucial role in the loading of curcumin onto the self-assembled nanoparticles. The kinetic stability of self-assembled nanoparticles studied using FRET shows a link between their integrity and cellular uptake in human glioblastoma cells. The present study demonstrates how minor changes in the molecular structure of gradient copoly(2-oxazoline)s can lead to significant differences in the loading, stability, cytotoxicity, cellular uptake, and pharmacokinetics of nano-formulations containing curcumin. The obtained results on the behavior of the complex of gradient copoly(2-oxazoline)s and curcumin may contribute to the development of effective next-generation polymeric nanostructures for biomedical applications.
Trvalý link: https://hdl.handle.net/11104/0338061