PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration

0568881 - ÚMCH 2024 RIV NZ eng J - Článek v odborném periodiku
Táborská, Johanka - Blanquer, Andreu - Brynda, Eduard - Filová, Elena - Stiborová, Lenka - Jenčová, V. - Havlíčková, K. - Riedelová, Zuzana - Riedel, Tomáš
PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration.
International Journal of Nanomedicine. Roč. 18, 3 February (2023), s. 595-610. E-ISSN 1178-2013
Grant CEP: GA MZd(CZ) NV18-01-00332; GA MŠMT(CZ) LX22NPO5104
Institucionální podpora: RVO:61389013 ; RVO:67985823
Klíčová slova: human platelet lysate * diabetic ulcer * fibrin coating
Obor OECD: Endocrinology and metabolism (including diabetes, hormones); Endocrinology and metabolism (including diabetes, hormones) (FGU-C)
Impakt faktor: 8, rok: 2022
Způsob publikování: Open access
https://www.dovepress.com/plclpcl-dressings-with-platelet-lysate-and-growth-factors-embedded-in--peer-reviewed-fulltext-article-IJN

The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ϵ-caprolactone) and poly(ϵ-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound.
Trvalý link: https://hdl.handle.net/11104/0340158