Surface effect of iron oxide nanoparticles on the suppression of oxidative burst in cells

0569400 - ÚMCH 2024 RIV US eng J - Článek v odborném periodiku
Swietek, Malgorzata Anna - Gunár, Kristýna - Kolodziej, A. - Weselucha-Birczynska, A. - Veverka, Pavel - Šebestová Janoušková, Olga - Horák, Daniel
Surface effect of iron oxide nanoparticles on the suppression of oxidative burst in cells.
Journal of Cluster Science. Roč. 34, č. 1 (2023), s. 323-334. ISSN 1040-7278. E-ISSN 1572-8862
Grant CEP: GA ČR(CZ) GC20-02177J
Institucionální podpora: RVO:61389013 ; RVO:68378271
Klíčová slova: oxidative stress * magnetic nanoparticles * immune cells
Obor OECD: Polymer science; Condensed matter physics (including formerly solid state physics, supercond.) (FZU-D)
Impakt faktor: 2.8, rok: 2022
Způsob publikování: Omezený přístup
https://link.springer.com/article/10.1007/s10876-022-02222-9

Overproduction of reactive oxygen species (ROS) is an unwanted phenomenon, leading to cellular damages. The aim of this study was to investigate the ability of neat and surface-modified iron oxide nanoparticles (IONs) to eliminate ROS produced by immune cells. The employed coating included heparin (ION@Hep) or heparin and chitosan grafted with phenolic compounds famous for antioxidant properties, i.e., gallic acid (ION@Ch-G) or phloroglucinol (ION@CH-P). A total peroxyl radical-trapping potential assay showed that both types of the phenolic compounds-modified IONs exhibited superior radical scavenging activity over the neat and ION@Hep particles at 100 μg/mL. Up to ~ 75 μg/mL, the particles were non-toxic towards RAW 264.7 macrophages. Capability of the particles to limit ROS production was investigated in vitro on polymorphonuclear (PMN) cells isolated from human whole blood and expressed as an ability to reduce the oxidative burst in the stimulated cells, as well as a potential to increase the viability of bacteria cultivated with the PMN cells. The highest viability of bacteria was observed for the neat and ION@Ch-G, while the ION@Ch-G particles also the most effectively inhibited the oxidative burst. The results indicated that ROS scavenging depend on the presence of polymer and selection of phenols, enriching the IONs.
Trvalý link: https://hdl.handle.net/11104/0340720