Heterosis versus breakdown in cyprinid hybrids associated with SVCV infection revealed by transcriptome profile analysis of head kidney

0575920 - ÚBO 2025 RIV NL eng J - Článek v odborném periodiku
Šimková, A. - Křížová, K. C. - Voříšková, K. - Vetešník, Lukáš - Hejret, V. - Gettová, L. - Vorel, J. - Reslová, N. - Bystrý, V.
Heterosis versus breakdown in cyprinid hybrids associated with SVCV infection revealed by transcriptome profile analysis of head kidney.
Aquaculture. Roč. 578, January (2024), č. článku 740083. ISSN 0044-8486. E-ISSN 1873-5622
Institucionální podpora: RVO:68081766
Klíčová slova: Carassius gibelio * Cyprinids * Cyprinus carpio * Hybrid breakdown * Hybrid heterosis * Hybridization * svcv * Transcriptome profile analysis
Obor OECD: Veterinary science
Impakt faktor: 4.5, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0044848623008578?via%3Dihub

Whilst the hybrids of F1 generations usually experience heterosis for fitness-related traits (including the resistance to parasites), post-F1 generations, due to Dobzhansky–Muller genetic incompatibilities, express numerous disadvantageous traits (including susceptibility to parasites). Genetic disruption in hybrids may also result from the broken system of cyto-nuclear coadaptation. Maternal backcrosses (having parents with the same mtDNA) and paternal backcrosses (having parents with different mtDNA) have the same nuclear genetic compositions, but differ in cytoplasmic genetic elements, affecting their viability and survival. Spring viraemia of the carp virus (SVCV), a disease with a serious economic impact in aquacultures, affects almost exclusively cyprinids, primarily common carp (Cyprinus carpio), and causes high mortality, whilst gibel carp (Carassius gibelio) is a less susceptible species. Our study was focused on the transcriptome profile analysis of head kidney to reveal differential gene expression in highly susceptible common carp, weakly susceptible gibel carp, and hybrid lines, hypothetizing that the patterns of differential gene expression will reflect hybrid heterosis in F1 generations and hybrid breakdown in backcrosses and F2 generations. We expected the differences in differential gene expression between maternal and paternal backcrosses to be in line with the hypothesis of broken cyto-nuclear coadaptation. The pattern of differential gene expression revealed from the transcriptomic analysis of head kidney was linked to the resistance or susceptibility to SVCV infections in two pure species, C. gibelio and C. carpio, and their various generations of inter-specific hybrids. We evidenced hybrid heterosis for F1 generations of hybrids, mostly reflected by similarity in the down-regulation of differentially expressed genes classified within the viral infection disease category. High hybrid breakdown was documented throughout the transcriptomic analysis for paternal backcross generations and F2 generation of hybrids, confirming the broken system of cyto-nuclear coadaptation in those post-F1 generations. This evidence may support the role of intrinsic selection acting against inter-specific hybridization in freshwater fish.
Trvalý link: https://hdl.handle.net/11104/0345610