Pitfalls of Mitochondrial Redox Signaling Research

0576981 - FGÚ 2024 RIV CH eng J - Článek v odborném periodiku
Ježek, Petr
Pitfalls of Mitochondrial Redox Signaling Research.
Antioxidants. Roč. 12, č. 9 (2023), č. článku 1696. E-ISSN 2076-3921
Grant CEP: GA ČR(CZ) GA21-01205S; GA MŠMT(CZ) LX22NPO5104
Institucionální podpora: RVO:67985823
Klíčová slova: redox signaling from mitochondria * matrix H2O2 release * H2O2 release into intracristal space * cytosolic H2O2 release * redox buffers * peroxiredoxins * redox-sensitive probes * MnSOD
Obor OECD: Physiology (including cytology)
Impakt faktor: 7, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2076-3921/12/9/1696

Redox signaling from mitochondria (mt) to the cytosol and plasma membrane (PM) has been scarcely reported, such as in the case of hypoxic cell adaptation or (2-oxo-) 2-keto-isocaproate (KIC) beta-like-oxidation stimulating insulin secretion in pancreatic beta-cells. Mutual redox state influence between mitochondrial major compartments, the matrix and the intracristal space, and the cytosol is therefore derived theoretically in this article to predict possible conditions, when mt-to-cytosol and mt-to-PM signals may occur, as well as conditions in which the cytosolic redox signaling is not overwhelmed by the mitochondrial antioxidant capacity. Possible peroxiredoxin 3 participation in mt-to-cytosol redox signaling is discussed, as well as another specific case, whereby mitochondrial superoxide release is diminished, whereas the matrix MnSOD is activated. As a result, the enhanced conversion to H2O2 allows H2O2 diffusion into the cytosol, where it could be a predominant component of the H(2)O(2 )release. In both of these ways, mt-to-cytosol and mt-to-PM signals may be realized. Finally, the use of redox-sensitive probes is discussed, which disturb redox equilibria, and hence add a surplus redox-buffering to the compartment, where they are localized. Specifically, when attempts to quantify net H2O2 fluxes are to be made, this should be taken into account.
Trvalý link: https://hdl.handle.net/11104/0346384