Synthesis and Application of Novel Fluorescent Labeling Tags for Oligosaccharide and N-Linked Glycan Analysis

0580627 - ÚIACH 2024 CZ eng A - Abstrakt
Veselý, H. - Greguš, M. - Pížová, H. - Smolková, Denisa - Čmelík, Richard - Lavická, Jana - Bobáľ, P.
Synthesis and Application of Novel Fluorescent Labeling Tags for Oligosaccharide and N-Linked Glycan Analysis.
Book of Abstracts. Prague: Czech Chemical Society, 2023. s. 1-1.
[Advances in Organic, Bioorganic and Pharmaceutical Chemistry /57./. 09.11.2023-11.11.2023, Špindlerův Mlýn]
Grant CEP: GA ČR(CZ) GA22-00236S
Institucionální podpora: RVO:68081715
Klíčová slova: oligosaccharide * novel fluorescent labeling tags
Obor OECD: Analytical chemistry
https://mab.uochb.cas.cz/liblice2023/programme.php

Monitoring glycosylation and investigating the influence of glycan structures on their function is an essential area of research, particularly in the diagnosis of disease and the study of mechanisms of pathogenesis. Analysis of glycans is still arduous because of their extreme structure complexity and conjugation diversity. But there are also instrumental aspects, such as the sensitivity limits of analyses. Therefore, glycan analysis is very often combined with fluorescence detection in addition to MS. In this work, we present the synthesis of novel fluorescent labeling tags for oligosaccharide and N-linked glycan analysis based on the hydrazide derivative of dipyrrometheneboron difluoride (BODIPY) and derivatives of 1,3-diaza-tricyclo[7.3.0.0³,⁷]dodeca-5,7,9,11-tetraene-2,4-dione.
Trvalý link: https://hdl.handle.net/11104/0349395