The translational oscillation in oocyte and early embryo development

0580751 - ÚŽFG 2024 RIV US eng J - Článek v odborném periodiku
Iyyappan, Rajan - Aleshkina, Daria - Ming, H. - Dvořan, Michal - Kakavand, Kianoush - Jansová, Denisa - del Llano, Edgar - Gahurová, Lenka - Bruce, A. W. - Mašek, T. - Pospíšek, M. - Horvat, Filip - Kubelka, Michal - Jiang, Z. - Šušor, Andrej
The translational oscillation in oocyte and early embryo development.
Nucleic Acids Research. Roč. 51, Nov 23 (2023), s. 12076-12091. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA ČR(CZ) GA22-27301S; GA MŠMT EF15_003/0000460
Institucionální podpora: RVO:67985904 ; RVO:68378050
Klíčová slova: translation * oocyte * early embryo
Obor OECD: Developmental biology; Biochemistry and molecular biology (UMG-J)
Impakt faktor: 14.9, rok: 2022
Způsob publikování: Open access
https://academic.oup.com/nar/article/51/22/12076/7405371?login=true

Translation is critical for development as transcription in the oocyte and early embryo is silenced. To illustrate the translational changes during meiosis and consecutive two mitoses of the oocyte and early embryo, we performed a genome-wide translatome analysis. Acquired data showed significant and uniform activation of key translational initiation and elongation axes specific to M-phases. Although global protein synthesis decreases in M-phases, translation initiation and elongation activity increases in a uniformly fluctuating manner, leading to qualitative changes in translation regulation via the mTOR1/4F/eEF2 axis. Overall, we have uncovered a highly dynamic and oscillatory pattern of translational reprogramming that contributes to the translational regulation of specific mRNAs with different modes of polysomal occupancy/translation that are important for oocyte and embryo developmental competence. Our results provide new insights into the regulation of gene expression during oocyte meiosis as well as the first two embryonic mitoses and show how temporal translation can be optimized. This study is the first step towards a comprehensive analysis of the molecular mechanisms that not only control translation during early development, but also regulate translation-related networks employed in the oocyte-to-embryo transition and embryonic genome activation.
Trvalý link: https://hdl.handle.net/11104/0349524