Omne vivum ex ovo: the oocyte reprogramming and remodeling activities

0580371 - ÚEM 2024 RIV GB eng J - Článek v odborném periodiku
Fulka, Helena - Loi, P. - Czernik, M. - Surani, A. - Fulka Jr, J.
Omne vivum ex ovo: the oocyte reprogramming and remodeling activities.
Reproduction. Roč. 165, č. 3 (2023), R75-R89. ISSN 1470-1626
Grant CEP: GA ČR(CZ) GF21-42225L
Institucionální podpora: RVO:68378041
Klíčová slova: topologically associating domains * zygotic genome activation * full-term development * nuclear transfer
Obor OECD: Reproductive biology (medical aspects to be 3)
Impakt faktor: 3.8, rok: 2022
Způsob publikování: Open access
https://rep.bioscientifica.com/view/journals/rep/165/3/REP-22-0124.xml

The mammalian oocyte is a unique cell, and comprehending its physiology and biology is essential for understanding fertilization, totipotency and early events of embryogenesis. Consequently, research in these areas influences the outcomes of various technologies, for example, the production and conservation of laboratory and large animals with rare and valuable genotypes, the rescue of the species near extinction, as well as success in human assisted reproduction. Nevertheless, even the most advanced and sophisticated reproductive technologies of today do not always guarantee a favorable outcome. Elucidating the interactions of oocyte components with its natural partner cell - the sperm or an 'unnatural' somatic nucleus, when the somatic cell nucleus transfer is used is essential for understanding how totipotency is established and thus defining the requirements for normal development. One of the crucial aspects is the stoichiometry of different reprogramming and remodeling factors present in the oocyte and their balance. Here, we discuss how these factors, in combination, may lead to the formation of a new organism. We focus on the laboratory mouse and its genetic models, as this species has been instrumental in shaping our understanding of early post-fertilization events.
Trvalý link: https://hdl.handle.net/11104/0351130