HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA

0585901 - ÚOCHB 2025 RIV US eng J - Článek v odborném periodiku
Benoni, Barbora - Potužník, Jiří František - Škríba, Anton - Benoni, Roberto - Trylčová, Jana - Tulpa, Matouš - Spustová, Kristina - Grab, K. - Mititelu, Maria Bianca - Pačes, Jan - Weber, Jan - Staněk, David - Kowalska, J. - Bednárová, Lucie - Kečkéšová, Zuzana - Vopálenský, Pavel - Gahurová, Lenka - Cahová, Hana
HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA.
ACS Chemical Biology. (2024). ISSN 1554-8929. E-ISSN 1554-8937
Grant CEP: GA MŠMT(CZ) LL1603; GA MŠMT(CZ) EH22_008/0004575
GRANT EU: European Commission(XE) 101041374 - StressRNaction
Výzkumná infrastruktura: e-INFRA CZ - 90140
Institucionální podpora: RVO:61388963 ; RVO:68378050
Impakt faktor: 4, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1021/acschembio.4c00151

Nicotinamide adenine dinucleotide (NAD) is a critical component of the cellular metabolism and also serves as an alternative 5′ cap on various RNAs. However, the function of the NAD RNA cap is still under investigation. We studied NAD capping of RNAs in HIV-1-infected cells because HIV-1 is responsible for the depletion of the NAD/NADH cellular pool and causing intracellular pellagra. By applying the NAD captureSeq protocol to HIV-1-infected and uninfected cells, we revealed that four snRNAs (e.g., U1) and four snoRNAs lost their NAD cap when infected with HIV-1. Here, we provide evidence that the presence of the NAD cap decreases the stability of the U1/HIV-1 pre-mRNA duplex. Additionally, we demonstrate that reducing the quantity of NAD-capped RNA by overexpressing the NAD RNA decapping enzyme DXO results in an increase in HIV-1 infectivity. This suggests that NAD capping is unfavorable for HIV-1 and plays a role in its infectivity.
Trvalý link: https://hdl.handle.net/11104/0353546