Počet záznamů: 1  

Lipid droplets control the negative effect of non-yeast sterols in membranes of Saccharomyces cerevisiae under hypoxic stress

  1. 1.
    0587471 - FGÚ 2025 RIV NL eng J - Článek v odborném periodiku
    Petrisková, L. - Kodedová, Marie - Balážová, M. - Sychrová, Hana - Valachovič, M.
    Lipid droplets control the negative effect of non-yeast sterols in membranes of Saccharomyces cerevisiae under hypoxic stress.
    Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1869, č. 7 (2024), č. článku 159523. ISSN 1388-1981. E-ISSN 1879-2618
    Grant CEP: GA MŠMT(CZ) LTC20006
    GRANT EU: European Commission(XE) CA18113 - EuroMicropH
    Institucionální podpora: RVO:67985823
    Klíčová slova: lipotoxicity * lipid droplet * fatty acid * sterol * plasma membrane * relative membrane potential
    Obor OECD: Microbiology
    Impakt faktor: 4, rok: 2023 ; AIS: 1.143, rok: 2023
    Web výsledku:
    https://doi.org/10.1016/j.bbalip.2024.159523DOI: https://doi.org/10.1016/j.bbalip.2024.159523

    The effectivity of utilization of exogenous sterols in the yeast Saccharomyces cerevisiae exposed to hypoxic stress is dependent on the sterol structure. The highly imported sterols include animal cholesterol or plant sitosterol, while ergosterol, typical of yeasts, is imported to a lesser extent. An elevated utilization of non-yeast sterols is associated with their high esterification and relocalization to lipid droplets (LDs). Here we present data showing that LDs and sterol esterification play a critical role in the regulation of the accumulation of non-yeast sterols in membranes. Failure to form LDs during anaerobic growth in media supplemented with cholesterol or sitosterol resulted in an extremely long lag phase, in contrast to normal growth in media with ergosterol or plant stigmasterol. Moreover, in hem1∆, which mimics anaerobiosis, neither cholesterol nor sitosterol supported the growth in an LD-less background. The incorporation of non-ergosterol sterols into the membranes affected fundamental membrane characteristics such as relative membrane potential, permeability, tolerance to osmotic stress and the formation of membrane domains. Our findings reveal that LDs assume an important role in scenarios wherein cells are dependent on the utilization of exogenous lipids, particularly under anoxia. Given the diverse lipid structures present in yeast niches, LDs fulfil a protective role, mitigating the risk of excessive accumulation of potentially toxic steroids and fatty acids in the membranes. Finally, we present a novel function for sterols in a model eukaryotic cell – alleviation of the lipotoxicity of unsaturated fatty acids.
    Trvalý link: https://hdl.handle.net/11104/0354638


     
     
Počet záznamů: 1  

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