Please use this identifier to cite or link to this item:
http://hdl.handle.net/11375/25302
Title: | Global Evaluation of the Escherichia coli Proteome during Stationary Phase |
Authors: | McFarlane, Nicole |
Advisor: | Schellhorn, Herb |
Department: | Biology |
Keywords: | E. coli;Proteomics;Stationary Phase;Long-term growth;Differential Expression;Tandem Mass Tags;Mass Spectrometry |
Publication Date: | 2019 |
Abstract: | Escherichia coli survives in both nutrient rich nutrient-limited environments. As such, understanding the gene and protein level activity that occurs during stationary phase is considered an important aspect of bacterial survival. Escherichia coli has been studied for decades providing substantial insight into gene expression profiles in exponential phase and recently, during adaptation to stationary phase. This led to the discovery of RpoS as a growth phase-dependent sigma factor. Further studies indicated that there are many genes that are expressed in an RpoS-independent but stationary phase-specific manner. However, proteins represent the functional molecules of the cell. Additionally, protein expression does not always correlate with the corresponding gene expression patterns. Therefore, to obtain an in depth understanding of the proteins that play a role in long-term growth in E. coli, TMT- (Tandem Mass Tags) based quantitative proteomic analysis was performed to identify proteins that are preferentially expressed during prolonged starvation. We identified proteins that were both positively and negatively regulated by RpoS during stationary phase, such as GadA and TnaA, respectively. RpoS levels peaked during early stationary phase and declined thereafter. However, proteins that were RpoS-dependent continued to increase during prolonged stationary phase. Additionally, we identified proteins that were expressed in an RpoS-independent manner during stationary phase. This suggests that protein expression during early stationary phase is distinct from prolonged stationary phase. Furthermore, RpoS-independent proteins may also play an important role during long-term growth. |
URI: | http://hdl.handle.net/11375/25302 |
Appears in Collections: | Open Access Dissertations and Theses |
Files in This Item:
File | Description | Size | Format | |
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McFarlane_Nicole_CA_2019May_Master of Science.pdf | 2.33 MB | Adobe PDF | View/Open |
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