Avaliação histopatológica da movimentação dentária induzida em molares de ratos submetidos à luxação extrusiva
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2016-12-20
Autores
Costa, Luciana Artioli [UNESP]
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Universidade Estadual Paulista (Unesp)
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Objetivo: Avaliar os efeitos da movimentação dentária induzida (MDI) em molares de ratos em função do tempo de reparo pós luxação extrusiva (LE). Métodos: Sessenta e três ratos machos adultos jovens (Rattus norvegicus albinus, Wistar), com 45 dias de idade e faixa de peso entre 230-250, foram distribuídos em nove grupos (n=7): grupos controle (C), trauma de LE e acompanhamento por 3, 5 ou 7 dias (T3D, T5D, T7D), movimentação controle (MC) – MDI por 7 dias,dispositivo controle (DC) – dispositivo de MDI inativo por 7 dias, e grupos submetidos a LE, espera por 3, 5 ou 7 dias e, então, submetidos a MDI por 7 dias (T3D/M, T5D/M, T7D/M). Após o período experimental os animais foram eutanasiados e cortes seriados longitudinais de 4μm, corados com hematoxilina e eosina, foram obtidos. Foram realizadas análises descritivas, semi-quantitativas e histomorfométricas do primeiro molar superior direito. A distribuição dos escores nos grupos foi comparada por meio do teste Kruskal Wallis com comparações múltiplas pelo método de Bonferroni e as análises histomorfométricas avaliadas pelo teste Kruskal Wallis, ambos com nível de significância de 5%. Resultados: Alterações vasculares (hemorragia) no ligamento periodontal e reabsorções radiculares foram observadas com maior frequência nos animais do grupo T3D/M, na face mesial da raiz disto-vestibular, com diferença significante (p<0.05) em relação aos controles. Conclusão: Quando o tempo de reparo do ligamento periodontal pós LE não é respeitado para o inicio da MDI, observa-se uma maior frequência de reabsorção radicular e alterações vasculares.
Objective: To evaluate the effects of induced tooth movement (ITM) on rat molars related to the time of repair after extrusive luxation (EL) trauma Methods: Sixty three male young adults rats (Rattus norvegicus Albinus, Wistar), 45 days old and weighing range of 230-250 were divided into nine groups (n = 7): control (C), EL trauma and follow-up for, 3, 5 or 7 days (T3D, GT5D, T7D), control movement (CM) – MDI for seven days, control device (CD) - MDI device inactive for 7 days, and groups submitted to LE, hold for 3, 5 or 7 days and, then, ITM for 7 days (T3D/M T5D/M T7D/M). After the experimental period, animals were euthanized and longitudinal serial sectioning of 4μm, stained with hematoxylin and eosin, were obtained. Descriptive, semi-quantitative and histomorfometrics analyzes of the right upper first molar were made. The distribution of scores in the groups was compared using the Kruskal Wallis test with multiple comparisons by Bonferroni method and histomorphometric analysis evaluated by Kruskal Wallis test, both with 5% significance level. Results: Vascular changes (bleeding) in the periodontal ligament and root resorption were observed more frequently in animals of T3D/M group, on mesial side of distobuccal root, with a significant difference (p<0.05) in relation to the controls. Conclusion: When the repair time of the periodontal ligament after LE is not respected for MDI beginning, a greater frequency of root resorption and vascular alterations is observed.
Objective: To evaluate the effects of induced tooth movement (ITM) on rat molars related to the time of repair after extrusive luxation (EL) trauma Methods: Sixty three male young adults rats (Rattus norvegicus Albinus, Wistar), 45 days old and weighing range of 230-250 were divided into nine groups (n = 7): control (C), EL trauma and follow-up for, 3, 5 or 7 days (T3D, GT5D, T7D), control movement (CM) – MDI for seven days, control device (CD) - MDI device inactive for 7 days, and groups submitted to LE, hold for 3, 5 or 7 days and, then, ITM for 7 days (T3D/M T5D/M T7D/M). After the experimental period, animals were euthanized and longitudinal serial sectioning of 4μm, stained with hematoxylin and eosin, were obtained. Descriptive, semi-quantitative and histomorfometrics analyzes of the right upper first molar were made. The distribution of scores in the groups was compared using the Kruskal Wallis test with multiple comparisons by Bonferroni method and histomorphometric analysis evaluated by Kruskal Wallis test, both with 5% significance level. Results: Vascular changes (bleeding) in the periodontal ligament and root resorption were observed more frequently in animals of T3D/M group, on mesial side of distobuccal root, with a significant difference (p<0.05) in relation to the controls. Conclusion: When the repair time of the periodontal ligament after LE is not respected for MDI beginning, a greater frequency of root resorption and vascular alterations is observed.
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Palavras-chave
Traumatismos dentários, Movimentação dentária, Modelos animais, Tooth injuries, Tooth movement, Models animal