Avaliação da influência de diferentes parâmetros na análise por microtomografia computadorizada (micro CTt) na doença periodontal induzida em ratos
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Data
2017-08-04
Autores
Sabino, Luana Elis [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Trabalhos in vivo têm utilizado a microtomografia computadorizada para avaliar a qualidade e quantidade de tecido ósseo presente durante o desenvolvimento de doença periodontal induzida. No entanto, ainda não foram estudados quais os melhores e mais confiáveis padrões estabelecidos para o adequado uso da técnica de Micro CT. O objetivo deste estudo foi avaliar a geração de artefatos e ruídos durante o escaneamento de biópsias ósseas bem como, analisar a influência de diferentes parâmetros estabelecidos durante a mensuração de área e volume ósseo, em modelo experimental de doença periodontal induzida em ratos. Para análise dos diferentes parâmetros de escaneamento com o Micro CT foram utilizados 05 animais aleatoriamente selecionados e submetidos a inserção de ligaduras na região subgengival dos segundos molares superiores. Após o período de 7 dias os animais foram eutanasiados e as biópsias ósseas escaneadas utilizando resoluções de 9 µm e 18 µm, com diferentes filtros: alumínio (0,2 mm, 0,5 mm e 1 mm) e cobre+alumínio (Cu 0,04 mm + Al 0,5 mm). Posteriormente foram definidas duas regiões de interesse (ROI’s) ao redor dos segundos molares, de modo que foi somente analisado o tecido ósseo. Após determinação da área de interesse, a quantidade de tecido ósseo foi avaliada e quantificada por meio do software CTAn, a partir de 5 valores de thresholds: 255-75, 255-80, 255-85, 255-70, 255-65. Quando diferentes tresholds foram analisados dentro de um mesmo filtro, somente o filtro de Al 0,2 mm apresentou diferença estatística para todas comparações (9 µm e 18 µm). Já quando diferentes filtros foram comparados com o mesmo treshold, apenas a comparação Al 0,2 mm x Cu + Al apresentou diferenças estatísticas para todas as comparações, com exceção para comparações entre Al 0,1 mm x Al 0,2 mm (treshold 255-65 com resolução de 18 µm). Diante dos resultados apresentados, conclui-se que, avanços e padronizações utilizadas em análises microtomográficas para a avaliação de perda óssea em doença periodontal induzida devem ser realizadas com objetivo de maior fidelização dos dados, podendo serem assim, compatíveis com outras análises com avaliações histomorfométricas.
In vivo studies have used a computerized microtomography (Micro CT) to assess a quality and amount of tissue present in the development of induced periodontal disease. However, have not yet studied which are the most reliable standards established for the proper use of the Micro CT technique. The objective of this study was to evaluate the generation of artifacts during biopsy scanning, as well as the analysis of different parameters defined in an area and bone volume measurement in an experimental model of periodontal disease induced in rats. To analyze the different detection parameters of microcomputers with selected animals and submitted to insertion of ligatures in the subgingival region of the upper second molars. After the 7-day period the animals were used as bone biopsies scanned using resolutions of 9 μm and 18 μm, with different filters: aluminum (0.2 mm, 0.5 mm and 1 mm) and copper + aluminum (Cu 0 04 mm + 0.5 mm). Subsequently, two regions of interest (ROI's) were defined around the second molars, in the way the bone tissue was analyzed. After determining the area of interest, the amount of bone tissue was evaluated and quantified using the CTAn software, from 5 threshold values: 255-75, 255-80, 255-85, 255-70, 255-65. When different thresholds were analyzed within the same filter, only the 0.2 mm Al filter presented statistical difference for all comparisons (9 μm and 18 μm). Already when the different filters with the same threshold were compared, it is only one comparison Al 0.2 mm x Cu + Al presented statistical difference for all comparisons, except for comparisons between Al 0.1 mm x 0.2 mm (threshold 255 - 65 with resolution of 18 μm). It was concluded that advances and standardization in microtomographic analysis to evaluate bone loss in induced periodontal disease should be performed with the objective of greater fidelity of the data, thus being compatible with other analyzes with histomorphometric evaluation.
In vivo studies have used a computerized microtomography (Micro CT) to assess a quality and amount of tissue present in the development of induced periodontal disease. However, have not yet studied which are the most reliable standards established for the proper use of the Micro CT technique. The objective of this study was to evaluate the generation of artifacts during biopsy scanning, as well as the analysis of different parameters defined in an area and bone volume measurement in an experimental model of periodontal disease induced in rats. To analyze the different detection parameters of microcomputers with selected animals and submitted to insertion of ligatures in the subgingival region of the upper second molars. After the 7-day period the animals were used as bone biopsies scanned using resolutions of 9 μm and 18 μm, with different filters: aluminum (0.2 mm, 0.5 mm and 1 mm) and copper + aluminum (Cu 0 04 mm + 0.5 mm). Subsequently, two regions of interest (ROI's) were defined around the second molars, in the way the bone tissue was analyzed. After determining the area of interest, the amount of bone tissue was evaluated and quantified using the CTAn software, from 5 threshold values: 255-75, 255-80, 255-85, 255-70, 255-65. When different thresholds were analyzed within the same filter, only the 0.2 mm Al filter presented statistical difference for all comparisons (9 μm and 18 μm). Already when the different filters with the same threshold were compared, it is only one comparison Al 0.2 mm x Cu + Al presented statistical difference for all comparisons, except for comparisons between Al 0.1 mm x 0.2 mm (threshold 255 - 65 with resolution of 18 μm). It was concluded that advances and standardization in microtomographic analysis to evaluate bone loss in induced periodontal disease should be performed with the objective of greater fidelity of the data, thus being compatible with other analyzes with histomorphometric evaluation.
Descrição
Palavras-chave
Microtomografia por Raios-X, Doenças periodontais, Osteogênese, Reabsorção óssea, X-ray microtomography, Periodontal diseases, Osteogenesis, Bone reabsorption