Contribution of ellagic acid on the antioxidant potential of medicinal plant Epilobium hirsutum

Date

2016-01-01

Author

Karakurt, Serdar
SEMİZ, ASLI
Celik, Gurbet
GENÇLER ÖZKAN, AYŞE MİNE
ŞEN, ALAATTİN
Adalı, Orhan

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In the present study, the possible role of ellagic acid (EA) on antioxidant potential of Epilobium hirsutum (EH) in rat liver was investigated. Wistar rats were intraperitoneally treated with 37.5 mg/kg of EH and 10 mg/kg of EA for 9 days. Effects of EH and EA on antioxidant [glutathione peroxidase (GPx) and superoxide dismutases (SOD)] and Phase II [NADPH quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTs)] enzyme activities, as well as protein and mRNA expressions of those, were investigated. Polyphenolic content of EH was determined by LC-MS/MS analysis. EH and EA injection to rats resulted in a significant increase of NQO1 (3.6-fold and 4.7-fold), GPx (1.45-fold), and SOD (1.34-fold and 1.27-fold) enzyme activities, whereas total GST (46% and 57%) and its isoforms, and GST mu (57% and 72%), and GST theta (60% and 68%) activities were significantly decreased. Western-blot and qRT-PCR analysis showed that NQO1 and GPx protein and mRNA expressions were increased significantly (P < 0.0001), whereas GST mu and GST theta were significantly decreased (P < 0.0001).

Subject Keywords

Glutathione-S-Transferase, Nad(P)H Quinone Oxidoreductase, Antiproliferative Activities, Dependent Bioactivation, Free-Radicals, Cells, Extracts, Ellagitannins, Resistance, Toxicity

URI

https://hdl.handle.net/11511/38473

Journal

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL

DOI

https://doi.org/10.1080/01635581.2016.1115092

Collections

Department of Biology, Article

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Citation Formats

S. Karakurt, A. SEMİZ, G. Celik, A. M. GENÇLER ÖZKAN, A. ŞEN, and O. Adalı, “Contribution of ellagic acid on the antioxidant potential of medicinal plant Epilobium hirsutum,” NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, pp. 173–183, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38473.