Inactivation and injury of Escherichia coli O157 : H7 and Staphylococcus aureus by pulsed electric fields

Date

2002-02-01

Author

DAMAR, SİBEL
Bozoglu, F
Hizal, MİRZAHAN
Bayındırlı, Alev

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Two pathogenic microorganisms Escherichia coli O157:H7 and Staphylococcus aureus, suspended in peptone solution (0.1% w/v) were treated with 12, 14, 16 and 20 kV/cm electric field strengths with different pulse numbers up to 60 pulses. Pulsed electric field (PEF) treatment at 20 kV/cm with 60 pulses provided nearly 2 log reduction in viable cell counts of E. coli O157:H7 and S. aureus. S. aureus cells were slightly more resistant than E.coli O157:H7 cells. The results related to the effect of initial cell concentration of E. coli O157:H7 on the PEF inactivation showed that more inactivation was obtained by decreasing initial cell concentration. Any possible injury by PEF was also investigated after applying 20 kV/cm electric field to the microorganisms. As a result, it was determined that there was 35.92 to 43.36% injury in E. coli O157:H7 cells, and 17.26 to 30.86% injury in S. aureus cells depending on pulse number. The inactivation results were also described by a kinetic model.

Subject Keywords

Electric field strength, S. aureus, Pulsed electric field, Pulse number, Pathogens, Modelling, Injury, Inactivation, E. coli O157 : H7

URI

https://hdl.handle.net/11511/43968

Journal

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

DOI

https://doi.org/10.1023/a:1013900222139

Collections

Department of Food Engineering, Article

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Citation Formats

S. DAMAR, F. Bozoglu, M. Hizal, and A. Bayındırlı, “Inactivation and injury of Escherichia coli O157 : H7 and Staphylococcus aureus by pulsed electric fields,” WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, pp. 1–6, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43968.