Bacterial anti-adhesive properties of a monolayer of zwitterionic block copolymer micelles

Date

2014-08-01

Author

Hippius, Catharina
Butun, Vural
Erel Göktepe, İrem

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

91
views

0
downloads

We report on bacterial anti-adhesive properties of a monolayer of block copolymer micelles (BCMs) with zwitterionic coronae and pH-responsive cores. BCMs were obtained by pH-induced self-assembly of selectively betainized poly[3-dimethyl (methacryloyloxyethyl) ammonium propane sulfonate-b-2-(diisopropylamino)ethyl methacrylate] (beta PDMA-b-PDPA) in aqueous solution above neutral pH. Monolayer films were self-assembled at pH 7.5 when beta PDMA-b-PDPA was in the micellar form. Bacterial anti-adhesive properties of the zwitterionic micellar coatings were examined against S. aureus through: i) a macroscopic test based on viable cell counting; ii) direct microscopic visualization of adherent bacteria by live/dead staining and iii) crystal violet staining to evaluate surface adherent biomass. 95% reduction in cell adhesion was observed by microscopy indicating the anti-adhesive properties of beta PDMA-b-PDPA micellar monolayer. Results obtained from the viable cell count assay and crystal violet staining showed similar trends and were in good agreement with the microscopy results. Such coatings are promising to impart both anti-adhesive and antimicrobial properties to a surface due to bacterial anti-adhesive properties of zwitterionic coronae and the potential of pH-responsive cores to release antimicrobial agents.

Subject Keywords

Mechanical Engineering, General Materials Science, Mechanics of Materials, Condensed Matter Physics

URI

https://hdl.handle.net/11511/47620

Journal

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

DOI

https://doi.org/10.1016/j.msec.2014.04.023

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Observation of in situ enhanced crystallization, negative resistance effect and photosensitivity in Tl2InGaSe4 crystals Qasrawi, A. F.; Irshaid, Tahani M. A.; Hasanlı, Nızamı (Elsevier BV, 2021-02-01) In this work, we report the properties of Tl2InGaSe4 crystals as multifunctional material. Namely, Tl2InGaSe4 crystals are grown by the modified Bridgman method using mixtures of TlInSe2 (50%) and TlGaSe2 (50%) single crystals. The enhanced crystallization and structural stabilities are monitored by the X-ray diffraction technique during the in situ heating and cooling cycles. The structural analyses on the Tl2InGaSe4 crystals revealed domination of both of the monoclinic and tetragonal phases in the crysta...
Thermally stimulated currents in n-InS single crystals Hasanlı, Nızamı; Yuksek, NS (Elsevier BV, 2003-03-24) Thermally stimulated current measurements are carried out on as-grown n-InS single crystals in the temperature range of 10-125 K. Experimental evidence is found for four trapping centers present in InS. They are located at 20, 35, 60 and 130 meV. The trap parameters have been determined by various methods of analysis, and they agree well with each other.
Electrical conductivity and Hall mobility in p-type TlGaSe2 crystals Qasrawi, AF; Hasanlı, Nızamı (Elsevier BV, 2004-07-02) Systematic dark electrical conductivity and Hall mobility measurements have been carried out in the temperature range of 200-350 K on p-type TlGaSe2 crystals. The analysis of the temperature-dependent electrical conductivity and carrier concentration reveals the extrinsic type of conduction with an acceptor impurity level located at 0.33 eV, and donor and acceptor concentrations of 9.0 x 10(15) and 1.3 x 10(16) cm(-3), respectively. A hole and electron effective masses of 0.520m(0) and 0.325m(0), respective...
Crystal data and indirect optical transitions in Tl2InGaSe4 crystals QASRAWI, ATEF FAYEZ HASAN; Hasanlı, Nızamı (Elsevier BV, 2008-06-03) The room temperature crystal data and the optical properties of the Bridgman method grown Tl2InGaSe4 crystals are reported and discussed. The X-ray diffraction technique has revealed that Tl2InGaSe4 is a single phase crystal of monoclinic structure. The unit cell lattice parameters, which were recalculated from the X-ray data, are found to be a = 0.77244 nm, b = 0.64945 nm, c = 0.92205 nm and beta = 95.03 degrees. The temperature dependence of the optical band gap of Tl2InGaSe4 single crystal in the tempera...
Tribological properties of MgO-CaO-SiO2-P2O5-F-based glass-ceramic for dental applications Park, Jongee; Öztürk, Abdullah (Elsevier BV, 2007-04-01) Microhardness and tribological behavior of a glass-ceramic produced from a glass in the MgO-CaO-SiO2-P2O5-F system have been investigated with regard to the phases formed and microstructure developed during crystallization. The results of X-ray diffraction analysis indicated that apatite and wollastonite are the predominant crystalline phases. Scanning electron microscopy examination revealed that the wollastonite crystal content decreases and apatite crystal content increases as the depth distance from the...

Citation Formats

C. Hippius, V. Butun, and İ. Erel Göktepe, “Bacterial anti-adhesive properties of a monolayer of zwitterionic block copolymer micelles,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 354–362, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47620.