Dielectrophoretic Spectra of CD133+/EpCAM+ HUH7 Cancer Stem Cells and HUH7 Cancer Cells

Date

2016-07-15

Author

Çağlayan, Zeynep
Demircan Yalçın, Yağmur
Özkayar, Gürhan
Kahraman, Deniz Cansen
Atalay, Rengül
Özgür, Ebru
Külah, Haluk

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URI

https://hdl.handle.net/11511/75471

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Dielectrophoretic Separation of K562 Human Leukemia Cells From Leukocytes by Isolated 3D Electrodes Bahrieh, Garsha; Özgür, Ebru; Külah, Haluk (null; 2014-09-03) This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DEP) platform for the separation and detection of the human leukemic cancer cells (K562) and its multidrug resistant (MDR) counterpart (K56/IMA-0.2 µM) from the human leukocyte mixture. The pro-posed DEP device, benefits from the 3D isolated electrodes inside a microfluidic channel. 3D elec-trodes (extruded 30 µm in z-direction) were isolated from the medium with thin parylene coating (~ 0.3 µm). In addition, the ...
Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system Demircan Yalçın, Yağmur; Toral, Taylan Berkın; Sukaş, Serkan; Yıldırım, Ender; Zorlu, Özge; Gündüz, Ufuk; Külah, Haluk (Chemical and Biological Microsystems Society (CBMS); 2019-10-27) This study presents label-free detection of imatinib resistance in K562 cells via integrated dielectrophoretic detection and impedimetric counting on a lab-on-a-chip system. Two impedimetric counting units were placed upstream and downstream of dielectrophoretic detection unit to obtain differential cell count and consequently the trapping ratio. The trapping ratio up to 57% was achieved for imatinib resistant (~60-fold) K562 cells, while it was minimized to 20% for wild type K562 cells
Dielectrophoretic cell chromatography device with spiral microfluidic channels and concentric electrodes, fabricated with MEMS technology Külah, Haluk (Türk Patent Enstitüsü, 2013-3-27) This dielectrophoretic micro cell chromatography device with concentric electrodes and spiral microfluidic channels, produced according to MEMS technology subject to this invention; is composed of 4 groups of effect electrodes, inlet electrodes, spiral zone and central span, having exterior upper electrode (1), interior sub electrode with 3D geometry (2), upper inlet electrode (3), sub inlet electrode (4), spiral zone (5), central span (6), constant reading point and Insulating wafer (7) as the main compone...
Dielectrophoretic Spectra of Polymorphonuclear White Blood Cells Çağlayan, Zeynep; Demircan Yalçın, Yağmur; Özkayar, Gürhan; Özgür, Ebru; Külah, Haluk (null; 2016-10-07)
Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes Bahrieh, G.; Koydemir, H. Ceylan; Erdem, Murat; Ozgur, E.; Gündüz, Ufuk; Külah, Haluk (2013-11-06) This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the ...

Citation Formats

Z. Çağlayan et al., “Dielectrophoretic Spectra of CD133+/EpCAM+ HUH7 Cancer Stem Cells and HUH7 Cancer Cells,” 2016, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/75471.