Amino Acid Substitutions in the Subunit Interface Enhancing Thermostability of Thermoplasma acidophilum Citrate Synthase

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1998-07-05

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Kocabıyık, Semra

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https://hdl.handle.net/11511/77806

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Amino acid substitutions in the subunit interface enhancing thermostability of Thermoplasma acidophilum citrate synthase Erduran, I; Kocabıyık, Semra (Elsevier BV, 1998-08-19) We have used citrate synthase from Thermoplasma (Tp.) acidophilum as a thermostable model system to investigate the role of hydrophobic interactions in dimer interface for maintaining high temperature stability. Three mutant enzymes were constructed by single amino acid substitutions in the interface helices: Ala97 --> Ser, Ala104 --> Thr, and Gly209 --> Ala. All of the mutations enhanced the thermostability of Tp. citrate synthase, while improving its catalytic properties (K-m, V-max, and specific activity...
Amino Acid Substitutions within the Analogous Nucleotide Binding Loop (P-Loop) of Aminoglycoside 3' -Phosphotransferase-II Kocabıyık, Semra (1994-01-01)
Amino acid substitution matrices based on 4-body Delaunay contact profiles Sacan, Ahmet; Toroslu, İsmail Hakkı (2007-10-17) Sequence similarity search of proteins is one of the basic and most common steps followed in bioninformatics research and is used in making evolutionary, structural, and functional inferences. The quality of the search and the alignment of the protein sequences depend crucially on the underlying amino-acid substitution matrix. We present a method for deriving amino acid substitution matrices from 4-body contact propensities of amino-acids in 3D protein structures. Unlike current popular methods, our method ...
Amino Acid Conjugated Spiropyrans: Synthesis and Photoisomerization Studies Şentürk, Bengi; Akdağ, Akın (2022-09-01) Among photo-switchable compounds, spiropyrans have attracted significant attention owing to their multi-responsiveness towards a change in the pH, solvent polarity, and temperature, along with light. Upon exposure to the external stimulants, the merocyanine form is obtained due to bond cleavage between spiro carbon and oxygen, which transforms the optically active spiropyrans into an achiral form. The back conversion of the resulting achiral merocyanine form to the spiropyrans brings about a racemic mixture...
AMINO-ACID SUBSTITUTIONS WITHIN THE ANALOGOUS NUCLEOTIDE-BINDING LOOP (P-LOOP) OF AMINOGLYCOSIDE 3'-PHOSPHOTRANSFERASE-II KOCABIVIK, S; PERLIN, MH (Elsevier BV, 1994-01-01) 1. Oligonucleotide-directed mutagenesis of APH(3')-II was used to investigate the functions of key amino acids in the P-loop analogous motif of the enzyme. 2. The mutations of Gly205 --> GIu, Gly210 --> Ala and Arg211 --> Pro considerably reduced the resistance of the resulting strains to KM and to related drugs, e.g. G418. 3. Similarly, enzyme activity in the crude extracts of these mutants was substantially reduced as well as the enzyme's affinity for Mg2+ ATP. 4. Alternatively substitutions at a highly c...

Citation Formats

S. Kocabıyık, “Amino Acid Substitutions in the Subunit Interface Enhancing Thermostability of Thermoplasma acidophilum Citrate Synthase,” 1998, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/77806.