The effect of cysteine 43 mutation on catalytic properties and thermostability of thermoplasma acidophilum citrate synthase

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1996

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Erduran, ipek

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https://hdl.handle.net/11511/8873

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Graduate School of Natural and Applied Sciences, Thesis

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The Effect of Cysteine 43 Mutation on Catalytic Properties and Thermostability of Thermoplasma acidophilum Citrate Synthase Kocabıyık, Semra (1996-07-07)
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The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum Kocabıyık, Semra; Russel, RJM; Danson, MJ; Hough, DW (Elsevier BV, 1996-07-05) In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutant Thermoplasma citrate synthase is very similar to wild-type citrate s...
The effect of valine substitution for glycine in the dimer interface of citrate synthase from Thermoplasma acidophilum on stability and activity Kocabıyık, Semra (Elsevier BV, 2000-08-28) To determine the role of hydrophobic interactions in the dimer interface of citrate synthase (CS) from Thermoplasma (Tp) acidophilum in thermostabilization, we have used site-directed mutagenesis to replace Gly 196 by Val on the helix L of the subunit interface. Recombinant wild-type and Gly 196 mutant TpCS enzymes were largely identical in terms of substrate specificities (K-m for oxaloacetate and acetyl CoA). However, the mutation not only reduced catalytic activity (about 10-fold) (i.e., V-max, K-cat and...
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Citation Formats

i. Erduran, “The effect of cysteine 43 mutation on catalytic properties and thermostability of thermoplasma acidophilum citrate synthase,” Middle East Technical University, 1996.