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Prospects of transcript profiling for mRNAs and microRNAs using formalin-fixed and paraffin-embedded dissected autoptic multiple sclerosis lesions

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Eisele,  Sylvia
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Krumbholz,  Markus
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Mohan,  Hema
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Junker,  Andreas
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Hohlfeld,  Reinhard
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Meinl,  Edgar
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Citation

Eisele, S., Krumbholz, M., Fischer, M.-T., Mohan, H., Junker, A., Arzberger, T., et al. (2012). Prospects of transcript profiling for mRNAs and microRNAs using formalin-fixed and paraffin-embedded dissected autoptic multiple sclerosis lesions. Brain Pathology, 22(5), 607-618. doi:10.1111/j.1750-3639.2012.00564.x.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-155B-5
Abstract
The elaboration of novel pathogenic aspects of multiple sclerosis (MS) requires the analysis of well-defined stages of lesion development. However, specimens of certain stages and lesion types are either present in small brain biopsies, insufficient in size for further molecular studies or available as formalin-fixed and paraffin-embedded (FFPE) material only. Therefore, application of current molecular biology techniques to FFPE tissue is warranted. We compared FFPE and frozen tissue by using quantitative polymerase chain reaction and report: (1) FFPE material is highly heterogeneous regarding the utility for transcript profiling of mRNAs; well-preserved FFPE samples had about a 100-fold reduced sensitivity compared with frozen tissue, but gave similar results for genes of sufficient abundance; (2) FFPE samples not suitable for mRNA analysis are still highly valuable for miRNA quantification; (3) the length of tissue fixation greatly affects utility for mRNA but not for miRNA analysis; (4) FFPE samples can be processed via a hot water bath for dissection of defined lesion areas; and (5) in situ hybridization for proteolipid protein (PLP) helps to identify samples not suitable for mRNA amplification. In summary, we present a detailed protocol how to use autoptic FFPE tissue for transcript profiling in dissected tissue areas.