High resolution shadowing of freeze-dried bacterial photosynthetic membranes: 
multivariate statistical analysis and surface relief reconstruction

Engelhardt, H.; Guckenberger, R.; Hegerl, R.; Baumeister, W.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

High resolution shadowing of freeze-dried bacterial photosynthetic membranes: multivariate statistical analysis and surface relief reconstruction

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Engelhardt, H., Guckenberger, R., Hegerl, R., & Baumeister, W. (1985). High resolution shadowing of freeze-dried bacterial photosynthetic membranes: multivariate statistical analysis and surface relief reconstruction. Ultramicroscopy, 16(3-4), 395-410.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-751A-A

Abstract

The surface reliefs of photosynthetic membranes from the bacterium Ectothiorhodospira halochloris have been reconstructed from shadowing data. Freeze-dried membranes were shadowed unidirectionally with tantalum/tungsten at low specimen temperature ( approximately 20K). The membranes, which contain the photosynthetic complexes hexagonally packed, show two distinctly different faces. Analysis of the apparent molecular displacement on the two faces suggests that not all features are compatible with P6 symmetry but may vary from one unit cell to another. Correspondence analysis and differential averaging created subsets reflecting the nature of the variability, namely an asymmetry and rotational variability of the reaction centre complex with respect to the surrounding ring of light-harvesting complexes. (16 References).