Functional genomics using high-throughput RNA interference

Vanhecke, Dominique; Janitz, Michal

doi:10.1016/S1359-6446(04)03352-5

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Functional genomics using high-throughput RNA interference

MPS-Authors

Vanhecke, Dominique
Max Planck Society;

/persons/resource/persons50363

Janitz, Michal
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

Vanhecke et al. - Drug Discov Today .pdf
(全文テキスト（全般）), 244KB

付随資料 (公開)

There is no public supplementary material available

引用

Vanhecke, D., & Janitz, M. (2005). Functional genomics using high-throughput RNA interference. Drug Discovery Today, 10(3), 205-212. doi:10.1016/S1359-6446(04)03352-5.

引用: https://hdl.handle.net/11858/00-001M-0000-0010-86E7-8

要旨

RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells. The review provides a comprehensive overview on current RNA interference-based high throughput genomic technologies. The cell array- and microwell plate-based loss-of-function cell assays are presented.