English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules

MPS-Authors
/persons/resource/persons15710

Riedel,  D.
Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14783

Antonin,  W.
Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14929

Fernandez-Chacon,  R.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15266

Jahn,  R.
Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

599514.pdf
(Publisher version), 594KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Riedel, D., Antonin, W., Fernandez-Chacon, R., de Toledo, G. A., Jo, T., Geppert, M., et al. (2002). Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules. Molecular and Cellular Biology, 22(18), 6487-6497. Retrieved from http://mcb.asm.org/cgi/reprint/22/18/6487.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-F30F-1
Abstract
Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D- deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.