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Journal Article

Released Fraction and Total Size of a Pool of Immediately Available Transmitter Quanta at a Calyx Synapse

MPS-Authors
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Schneggenburger,  R.
Research Group of Synaptic Dynamics and Modulation, MPI for Biophysical Chemistry, Max Planck Society;

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Meyer,  A. C.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Neher,  E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Schneggenburger, R., Meyer, A. C., & Neher, E. (1999). Released Fraction and Total Size of a Pool of Immediately Available Transmitter Quanta at a Calyx Synapse. Neuron, 23(2), 399-409. doi:10.1016/S0896-6273(00)80789-8.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-FB9B-0
Abstract
The size of a pool of readily releasable vesicles at a giant brainstem synapse, the calyx of Held, was probed with three independent approaches. Using simultaneous pre- and postsynaptic whole-cell recordings, two forms of presynaptic Ca2+ stimuli were applied in rapid succession: uncaging of Ca2+ by flash photolysis and the opening of voltage-gated Ca2+ channels. The ensuing transmitter release showed a nearly complete cross-inhibition between the two stimuli, indicating the depletion of a limited pool of about 700 transmitter quanta. The pool size was confirmed in experiments using enhanced extracellular Ca2+ concentrations, as well as short, high-frequency stimulus trains. The results reveal a surprisingly large pool of functionally available vesicles, of which a fraction of about 0.2 is released by a single presynaptic action potential under physiological conditions.