Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose 
autoradiography

Rodrigues, V; Bülthoff, I

doi:10.1016/0165-0270(85)90066-4

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_1794919_2

DetailsSummary

Released

Journal Article

Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography

MPS-Authors

/persons/resource/persons83840

Bülthoff, I
Department Human Perception, Cognition and Action, Max Planck Institute for Biological Cybernetics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Rodrigues, V., & Bülthoff, I. (1985). Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography. Journal of Neuroscience Methods, 13(3-4), 183-190. doi:10.1016/0165-0270(85)90066-4.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0013-F005-D

Abstract

High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at −76°C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.