Intermediate dipolar distances from spin labels.

Marsh, D.

doi:10.1016/j.jmr.2013.11.001

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Intermediate dipolar distances from spin labels.

MPS-Authors

/persons/resource/persons15495

Marsh, D.
Emeritus Group of Spectroscopy and Photochemical Kinetics, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/S109078071300284X/pdfft?md5=09f2b18ade6424e810a2b6df5bc9b4fe&pid=1-s2.0-S109078071300284X-main.pdf
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

1921605.pdf
(Publisher version), 397KB

Supplementary Material (public)

There is no public supplementary material available

Citation

Marsh, D. (2014). Intermediate dipolar distances from spin labels. Journal of Magnetic Resonance, 238, 77-81. doi:10.1016/j.jmr.2013.11.001.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-3677-0

Abstract

Methods for determining inter-spin distances between nitroxide spin labels from dipolar couplings in the intermediate range (r(12) approximate to 1.1-2 nm) by CW-EPR are addressed. For nitroxide powder patterns, the assumption of unlike spins is a better approximation than assuming strong coupling between like spins. Methods that determine the average splitting in dipolar deconvolutions yield the mean value < 1/r(12)(3)>, and those that determine the dipolar contribution to the spectral second moment correspondingly yield < 1/r(12)(6)>. To relate these ensemble averages to the mean inter-spin distance < r(12)> requires knowledge of the distribution in r(12). Values of the inverse roots r(eff,n) = < 1/r(12)(n)>(-1/n) always lie below < r(12)>. Consistent comparisons of literature data on double-labelled alpha-helical peptides support this view. Evaluations of r(eff,n) for Gaussian distributions in r(12) yield calibrations to determine < r(12)> for a given distribution width, sigma(r12).