Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron 
Tomograms

Fernandez, Jose-Jesus; Laugks, Ulrike; Schaffer, Miroslava; Bäuerlein, Felix J. B.; Khoshouei, Maryam; Baumeister, Wolfgang; Lucic, Vladan

doi:10.1016/j.bpj.2015.10.043

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Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms

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Laugks, Ulrike
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Schaffer, Miroslava
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Bäuerlein, Felix J. B.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons127963

Khoshouei, Maryam
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77721

Baumeister, Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78340

Lucic, Vladan
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Fernandez, J.-J., Laugks, U., Schaffer, M., Bäuerlein, F. J. B., Khoshouei, M., Baumeister, W., et al. (2016). Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms. BIOPHYSICAL JOURNAL, 110(4), 850-859. doi:10.1016/j.bpj.2015.10.043.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002A-267F-2

Abstract

Imaging of fully hydrated, vitrified biological samples by electron tomography yields structural information about cellular protein complexes in situ. Here we present a computational procedure that removes artifacts of three-dimensional reconstruction caused by contamination present in samples during imaging by electron microscopy. Applying the procedure to phantom data and electron tomograms of cellular samples significantly improved the resolution and the interpretability of tomograms. Artifacts caused by surface contamination associated with thinning by focused ion beam, as well as those arising from gold fiducial markers and from common, lower contrast contamination, could be removed. Our procedure is widely applicable and is especially suited for applications that strive to reach a higher resolution and involve the use of recently developed, state-of-the-art instrumentation.