Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET 
control.

Hasan, Mazahir T.; Friedrich, Rainer W.; Euler, Thomas; Larkum, Matthew E.; Giese, Günter; Both, Matthias; Dübel, Jens; Waters, David Jack; Bujard, Hermann; Griesbeck, Oliver; Tsien, Roger Y.; Nagai, Takeharu; Miyawaki, Atsushi; Denk, Winfried

doi:10.1371/journal.pbio.0020163

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Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.

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Hasan, Mazahir T.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Friedrich, Rainer W.
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92839

Euler, Thomas
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94010

Larkum, Matthew E.
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons118183

Giese, Günter
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92290

Both, Matthias
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92745

Dübel, Jens
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95861

Waters, David Jack
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons128986

Denk, Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://dx.doi.org/10.1371/journal.pbio.0020163
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Citation

Hasan, M. T., Friedrich, R. W., Euler, T., Larkum, M. E., Giese, G., Both, M., et al. (2004). Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biology, 2(6): e163, pp. 763-775. doi:10.1371/journal.pbio.0020163.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002A-26D8-7

Abstract

Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.