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Helper T-cell Phenotypes and Related Cytokine Gene Expression in Chronic Graft-versus-host Disease

URL to cite or link to: http://hdl.handle.net/1802/30979

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Thesis (Ph.D.)--University of Rochester. School of Nursing. Dept. of Health Practice Research, 2016.
Chronic graft-versus-host disease (cGVHD) is a common life threatening complication of allogeneic stem cell transplant (SCT). Although the pathophysiology of cGVHD is theorized to involve donor-derived adaptive T-cell responses, there has been limited translation of this understanding to biomarkers that aid in the diagnosis and management of cGVHD. Biomarkers will assist in diagnosis of cGVHD which is currently based on clinical symptoms and corroborated by histopathology. Biomarkers will also be instrumental in the early diagnosis of cGVHD and perhaps prior to the development of disease manifestations. Chronic GVHD can be described as an allo-mediated, autoimmune-like phenomenon involving cytokines and resultant cellular responses. Helper T-cells are known to play a role in some autoimmune diseases that share clinical features of cGVHD, specifically, Th1, Th2, Th17 and regulatory T-cells. The immune events that contribute to the development of cGVHD are presumed to occur early after SCT. The results of this study include that these four helper T-cell populations were rare at 60 days after SCT when measured by standard procedures for flow cytometry and single subtype surface markers, thus limiting our ability to explore related cytokine relative gene expression utilizing standard polymerase chain reaction technology. Our findings further highlight the need for research directed toward cGVHD pathophysiology and biomarker discovery.
Contributor(s):
Jamie L. Oliva - Author

Susan Groth - Thesis Advisor

Primary Item Type:
Thesis
Language:
English
Subject Keywords:
T-cell; Helper T-cell; Chronic graft-versus-host disease
Sponsor - Description:
Oncology Nursing Foundation - Dissertation award
Sigma Theta Tau International Epsilon Xi Chapter - Research Grant
First presented to the public:
6/22/2016
Originally created:
2016
Original Publication Date:
2016
Previously Published By:
University of Rochester
Place Of Publication:
Rochester, N.Y.
Citation:
Extents:
Illustrations - some color
Number of Pages - xv, 127 pages
License Grantor / Date Granted:
Jennifer McCarthy / 2016-06-22 09:18:44.017 ( View License )
Date Deposited
2016-06-22 09:18:44.017
Date Last Updated
2022-09-26 21:01:02.388047
Submitter:
Jennifer McCarthy

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