Title:

High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments

Advisor: Simmons, Craig
Issue Date: Mar-2013
Abstract (summary): The process of finding a new drug against a chosen target for a vascular disease usually involves high-throughput screening in static multi-well plates. However, due to the disconnect between cell behavior in static culture and the physiological environment, only a small fraction of move on to clinical trials. To address this, I designed and fabricated a multi-channel microfluidic platform capable of mimicking key vascular environments to be used for drug screening. An experiment studying the effects of TNFα on moncyte-EC adhesion were performed under flow conditions using the platform and in a static multi-well plate. Results showed a similar response in monocyte adhesion with the increase in TNFα dose in both conditions. However, wells with non-treated ECs in static plates produced 40x more florescence than wells with no monocytes. The microfludic platform however circumvents this issue by washing away these weakly adhered ECs allowing small dose variations to be noticeable.
Content Type: Thesis

Permanent link

https://hdl.handle.net/1807/69980

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