New Tools for High Throughput Chemistry and Biology
Date
2011Author
Galveia, Luciano Paulo Rosa
Metadata
Abstract
New strategies for the high-throughput determination of protease substrate
specificity using a dual colour microarray based format with a small library of
FRET-based peptides were developed. Integral to this process was the development
of an in-house hydrophobic microarray platform and the application of microarrays
of microwells. Overall this allowed the rapid and quantitative analysis of a range of
peptides with three proteases, subtilisin, chymopapain and thermolysin.
An immobilised tetrafluoroarylsulfonyl chloride linker was developed to be
used as a traceless linker for microwave-assisted palladium chemistry, namely
transfer hydrogenation and deuteration, and Heck, Stille, Suzuki-Miyaura and
Buchwald-Hartwig cross-coupling reactions. Palladium-mediated protocols under
microwave irradiation were performed, allowing transfer hydrogenation and
deuteration, and Suzuki-Miyaura and Heck cross-couplings.