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http://hdl.handle.net/1843/SSLA-7VGJ5D| Type: | Dissertação de Mestrado |
| Title: | Produção e caracterização de anticorpos monoclonais anti épsilon toxina de Clostridiun perfrigens tipo D |
| Authors: | Theonys Diogenes Freitas |
| First Advisor: | Francisco Carlos Faria Lobato |
| First Co-advisor: | Luiz Guilherme Dias Heneine |
| First Referee: | Isabella Bias Fortes Ferraz |
| Second Referee: | Zelia Ines Portela Lobato |
| Abstract: | Foram produzidos anticorpos monoclonais contra épsilon toxina de Clostridium perfringens tipo D, a partir da fusão das linhagens de mieloma Sp2/0. Ag14 e P3-X63-Ag8. 653 com células do baço de camundongos BALB/c imunizado com o toxóide épsilon. Os números de wells positivos foram 59 para linhagem Sp2/0. Ag14 e 19 para P3-X63-Ag8. 653, representando 6,68 e 6,59% de positividade respectivamente. Seis linhagens de híbridos secretores de AcMo das classes e IgM () e IgG subclasse IgG3 () foram estabelecidas. A partir do sobrenadante da cultura dos híbridos foi possível obter-se uma média de 21,52 g/mL de IgM e 15 g/mL de IgG3 anti épsilon toxina de Clostridium perfringens tipo D |
| Abstract: | Monoclonal antibodies reactive against épsilon toxin from Clostridium perfringens type D were produced. Myeloma cell lines Sp2/0. Ag14 and P3-X63-Ag8.653 were fused with spleen cells from mice BALB/c immunized with epsilon toxoid. After fusion 59 wells were positive for the Sp2/0. Ag14 cell line and 19 for the P3-X63-Ag8.653, representing 6.68 and 6.59% respectively. Six hybrids were established secreting MoAb of the IgM (k) class and IgG3 (k) subclass. An average of 21.52 g/ml of IgM and 15 g/ml of IgG3 anti-epsilon toxin MoAb were obtained from the hybrids culture supernatant |
| Subject: | Anticorpos monoclonais Clostridium perfringens Toxinas |
| language: | Português |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| Rights: | Acesso Aberto |
| URI: | http://hdl.handle.net/1843/SSLA-7VGJ5D |
| Issue Date: | 3-Apr-2006 |
| Appears in Collections: | Dissertações de Mestrado |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| disserta__o_theonys.pdf | 191.23 kB | Adobe PDF | View/Open |
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