Genetic regulation in Clostridium acetobutylicum ATCC 824
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Abstract
Genetic Regulation in Clostridium acetobutylicum was studied to understand what signals caused this bacterium to switch between different growth stages. A biochemical approach was employed to study DNA supercoiling, while a molecular approach was employed to study sigma factors.
Cells treated with novobiocin, a gyrase inhibitor, produced higher butyrate levels and lower butanol and acetone levels. Seven enzyme activities involved in acid and solvent production were tested; CoA transferase, which catalyzes acetone formation and acid uptake, experienced a 50% decrease in activity. However, Northern analysis showed that CoA transferase mRNA levels did not decrease to a higher extent than mRNA levels of other enzymes involved in acid and solvent production, suggesting that the regulation was at the post-transcriptional level. The data also suggest that acetone and alcohol production may be regulated by different mechanisms.
Three genes were cloned from C. acetobutylicum into Escherichia coli and sequenced. Two of them encoded proteins that show high homology with two Bacillus subtilis sporulation-specific sigma factors,
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Citation
Wong, John. "Genetic regulation in Clostridium acetobutylicum ATCC 824." (1995) Diss., Rice University. https://hdl.handle.net/1911/16899.