Graduate Thesis Or Dissertation
 

Microbial diversity of sulfide structures from hydrothermal vent sites at 9°N, Guaymas Basin and the Juan de Fuca Ridge

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  • The microbial diversity associated with sulfide structures from three different hydrothermal vent fields was examined using phylogenetic analysis based on the small subunit ribosomal RNA gene (16S rDNA). Samples were collected from sites at 9°N, Guaymas Basin and the Juan de Fuca Ridge and analyzed with denaturing gradient gel electrophoresis (DGGE). In addition, clone libraries were constructed for two samples from Guaymas Basin. The Bacteria from Juan de Fuca Ridge all clustered within the e-Proteobacteria, while the Archaea identified from all three sites were Euryarchaeota. Among the Euryarchaeota were sequences clustering within the Methanococcales, Methanosarcinales, Methanobacteriales, and Methanomicrobiales; the later two groups have not previously been identified as part of the microbial community at deep-sea hydrothermal vents. Two groups of sequences were identified that cluster with sequences previously only identified from hydrothermal systems in the western Pacific and the Mid-Atlantic Ridge; these groups may be endemic to deep-sea hydrothermal vents.
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