Process for producing a cloned luciferase-synthesizing microorganism
Abstract
A process for producing a cloned luciferase-synthesizing microorganism, capable of the expression of bioluminescence, is disclosed. The process involves isolation and digestion of the genomic DNA of an appropriate bioluminescent microorganism, such as Vibrio harveyi, to obtain a DNA fragment which codes for luciferase. The DNA fragment is inserted into a pre-existing cloning vehicle under the control of an expression promoter, thereby producing a recombinant or derivatized cloning vehicle. A host microorganism is transformed with the derivatized cloning vehicle, cultured and purified for the expression of bioluminescence.
Subject
435/6.13435/189
435/252.3
435/252.33
435/320.1
435/488
435/849
536/23.1
536/23.2
C12N 9/0071
C12Y 114/14003
Y10S 435/849
Collections
Citation
Baldwin, Thomas O. (1986). Process for producing a cloned luciferase-synthesizing microorganism. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /176686.