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Mapping of genes using a bovine BAC Library to determine their effects on economically important traits in cattle
Abstract
Five genes, growth hormone (GH), insulin-like growth factor I receptor (IGF-1R), somatostatin (SS), prolactin (PRL) and placental lactogen (PLL) were isolated from a bovine bacterial artificial chromosome (BAC) Library. The BAC clones containing GH, IGF-I R, SS, PRL and PLL were physically mapped by flourescence in situ hybridization to chromosomes 19, 21, 1, 23 and 23 respectively. The BAC clones were screened for microsatellites. A single microsatellite was found for IGF-1R, two for SS, one for GH and none were identified for PRL and PLL. The 'Angelton' resource animals which consisted of 78 parents and grandparents, 31 backcross full-sib families produced by F, Angus-Brahman animals and straightbred Angus and Brahman animals (n=3 5 1) and 2 F2 families from F, Angus-Brahman animals (n=28) were genotyped for the identified microsatellites. Phenotypic data was collected for birth weight (BW), cannon bone length and circumference (CBL and CBC), gestation length (GEST), weaning weight and height (WWT and "T), slaughter weight and height (SWT and SLHT), hot carcass weight (HCWT), rib-eye area (REA), kidneypelvic-heart fat (KPH), adjusted fat thickness (ADJ), marbling (MARB) and quality and yield grade (QG and YG). The GH microsatellite (KLHGHM) was polymorphic in the Angleton families and was analyzed for association with significant differences among calves for the phenotypic traits measured. Results of the analyses showed that genotype at KLHGHM had an effect on KPH and SLHT (P<. 0 1). The analyses did not show the genotype at KLHGHM to have an effect on any of the other traits measured (P>.05). Sex of calf was an important source of variation for all traits except REA, MARB and QG. Sire breed by dam breed combinations were significant for BW, CBL, GEST, WHT, SHT and QG and the interaction between these combinations and sex of calf were also significant for BW, CBC, SWT, HCWT and YG. Regression on age was significant for variation of WWT and WHT, but not for the slaughter traits. Season-year of birth was significant for CBL, CBC, WWT, WHT, HCWT and SLHT. Family within sire breed by dam breed combinations was highly significant (P<.005) for BW, CBL, CBC, GEST, WWT, WHT, and all slaughter traits except YG.
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Citation
Herring, Kimberly Lynn (1995). Mapping of genes using a bovine BAC Library to determine their effects on economically important traits in cattle. Master's thesis, Texas A&M University. Available electronically from https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1995 -THESIS -H47.
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