Abstract
Peach [Prunus persica (L.) Batch] has been proposed as a model organism for woody perennials. To facilitate genomic research in peach, a binary bacterial artificial chromosome (BIBAC) library of var. Texking was constructed using a plant-transformation-competent vector, pCLD0451. Texking is a medium chill peach used in the Texas A&M University Prunus breeding program that displays valuable agronomic traits. The library was constructed in the BamH I site of the pCLD0451 vector and arrayed in 40 384-well microplates. It is comprised of 15,360 clones, having an average insert size of 123.6 kb. Of the 15,360 clones in the library, 768 (5%) were found to have no inserts. Screening with chloroplast probes from barley indicated that 32 (0.22%) of the library originated from the chloroplast genome. Therefore, 14,557 clones of the library originated from the nuclear genome, representing 6.8 X haploid genomes of the species. The BIBAC library was further screened with 8 RFLP markers selected from the peach genetic map. Library screening with the markers showed an average of 4.5 positive clones per marker, with a range of 1 to 11 positive clones. The library represents the first plant-transformation-competent BIBAC library for P. persica and will facilitate genomic research in peach and related species.
Wakefield, Laura (2002). Construction and characterization of a peach binary bacterial artificial chromosome library. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -2002 -THESIS -W234.