Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.12530/55712
Title: | Prevalence, detection and characterisation of fosfomycin-resistant Escherichia coli strains carrying fosA genes in Community of Madrid, Spain. | |
Authors: | ||
Filiation: | ||
Keywords: | ||
Mesh: | ||
Issue Date: | 21-Mar-2021 | |
Citation: | J Glob Antimicrob Resist. 2021;25:137-141 | |
Abstract: | The aim of this this study was to describe the presence of different variants of the fosA gene in fosfomycin-resistant Escherichia coli strains in Madrid, Spain. fos genes were searched for in 55 E. coli strains collected from seven representative hospitals located in Madrid. A phenotypic screening test was performed following the disk diffusion method with sodium phosphonoformate added as described by Nakamura et al. Additionally, a molecular study based on PCR was used to confirm the screening results. Positive strains for fos genes were further subjected to whole-genome sequencing (WGS). Phenotypic screening was positive in 9/55 strains (16.4%), although genotypic detection was positive in only 3 (fosA3, fosA4 and fosA6). Thus, the prevalence of fos genes in Madrid was 5.5% (3/55). WGS data were not available for the fosA6-positive strain. One isolate with fosA3 (ST69) carried a blaCTX-M-55 gene and seven virulence genes (air, eilA, iha, iss, lpfA, sat and senB). The fosA4-positive isolate (ST4038) carried the virulence genes iss, lpfA, iroN and mchF. Both fos genes were located between two IS26 mobile elements of a plasmid. We detected the presence of different variants of plasmid-mediated fosA genes in fosfomycin-resistant E. coli strains in Madrid, Spain. Despite the few reports in Europe, it would be of interest to monitor the spread of these acquired resistance genes. | |
PMID: | 33757820 | |
URI: | https://hdl.handle.net/20.500.12530/55712 | |
Rights: | openAccess | |
Appears in Collections: | Fundaciones e Institutos de Investigación > IIS H. U. Getafe > Artículos Fundaciones e Institutos de Investigación > FIIB H. U. Infanta Sofía y H. U. Henares > Artículos Hospitales > H. U. Infanta Sofía > Artículos | |
Files in This Item:
File | Size | Format | |
---|---|---|---|
Loras_2021_Prevalence detection characterisation.pdf | 927.08 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License