Avaliação de um novo kit (TF-TEST) nacional destinado ao diagnostico de enteroparasitoses em amostras fecais
Jancarlo Ferreira Gomes
DISSERTAÇÃO
Português
(Broch.)
T/UNICAMP G585a
Campinas, SP : [s.n.], 2004.
Orientadores: Luiz Candido de Souza Dias e Sumie Hoshino Shimizu
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Resumo: As enteroparasitoses humanas constituem atualmente um dos problemas de saúde pública tanto para países em desenvolvimento como para países desenvolvidos, em virtude do aumento no número de viajantes a regiões endêmicas e principalmente por causa de indivíduos imunocomprometidos. ...
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Resumo: As enteroparasitoses humanas constituem atualmente um dos problemas de saúde pública tanto para países em desenvolvimento como para países desenvolvidos, em virtude do aumento no número de viajantes a regiões endêmicas e principalmente por causa de indivíduos imunocomprometidos. Devido aumentar a probabilidade do encontro de organismos pelo exame de amostras múltiplas, pelo menos três exames parasitológicos das fezes são necessários, a fim de obter resultados sensíveis. Desta forma, idealizamos e desenvolvemos um kit (TF-Test) prático e econômico, agora em vias de ser fabricado em escala industrial, por uma empresa (Immunoassay Ind. Com. Ltda., São Paulo, Brasil). Este kit possibilita, em dias alternados, a coleta de três amostras fecais, separadamente, em tubos contendo uma solução preservadora. No laboratório, as três amostras são processadas em uma só etapa, utilizando um conjunto, onde a mistura passa por duplo filtro, e é concentrada por uma centrifugação rápida. O presente trabalho trata sobre avaliações dos protótipos do kit realizadas em duas fases, sendo a primeira, intralaboratorial abrangendo um período de seis meses, e a segunda, interlaboratoriais de dois anos. Na primeira fase, a técnica de TF-Test foi avaliada no estudo de 30 pacientes com parasitoses intestinais e 30 sem infecção parasitária, obtendo uma sensibilidade de 96,6%, significativamente (p < 0,05) maior que as demais técnicas convencionais comerciais (Coprotest, StillQuick e Parasep) ou não (Lutz ou Hoffman, Pons & Janer). Ademais, a técnica em questão apresentou um elevado índice de concordância k (0,920), considerado como Quase Perfeito, diferindo das técnicas convencionais. Em vista do potencial uso do protótipo para finalidade diagnóstica, o trabalho teve continuidade na segunda fase, onde os protótipos aprimorados foram avaliados no estudo de 1.244 pacientes em seis diferentes laboratórios de setor público e privado, considerados como sendo de referência ou altamente conceituados. Os dados obtidos demonstram que a técnica de TF-Test é de fato melhor que as técnicas convencionais utilizadas (Coprotest, Lutz e associação de Lutz, Faust & Cols. e Rugai, Mattos & Brisola), por apresentar sensibilidades que variam de 82,6% a 100%, estatisticamente maiores (p < 0,05) que as técnicas convencionais. Em quatro laboratórios, o índice k de TF-Test teve a classificação de Quase Perfeito e em dois laboratórios, Substancial. A alta sensibilidade e os aspectos econômicos e práticos demonstram que a técnica de TF-Test é apropriada para diagnóstico individual, inquéritos populacionais, assim como, para avaliação de quimioterapia instituída em programas de controle das parasitoses intestinais
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Abstract: Human intestinal parasitoses constitute a serious public health problem either in developing or developed countries. Those enteroparasitoses could be attributed to the increase of travelers around endemic areas and mainly due to immunocompromised patients.
In order to obtain... Ver mais Abstract: Human intestinal parasitoses constitute a serious public health problem either in developing or developed countries. Those enteroparasitoses could be attributed to the increase of travelers around endemic areas and mainly due to immunocompromised patients.
In order to obtain sensible results and to have a high chance to find organisms by multiple sample exams, it is necessary at least three serial parasitologycal faecal tests. For these reasons, a practice and non-expensive kit (TF-Test) was developed for being industrialized by a specialized company (Immunoassay Ind. Com. Ltda., Sao Paulo, Brazil). This kit makes possible to carry out three different faecal samples each other day. The three faecal samples are separately collected into tubes containing a preservative solution. Laboratory evaluation is done in just one step by using a pool of the three faecal samples. Mixture mass is passing by a double filter and then centrifuged for concentrating the mixture. Thus, the present study reports about evaluations of a prototype kit developed into two different phases: first phase was a six months stage inside developer laboratory and, the second one was a two years stage involving six different collaborative laboratories. In the first phase, parasitologycal exams using TF-Test was carried out in 30 patients with intestinal parasites and in 30 patients free of intestinal parasites and, it was significantly more sensible (96,6%, p<0,05) compared with conventional techniques as Coprotest, StillQuick e Parasep or with those frequently used as Lutz or Hoffman, Pons & Janer. Furthermore, TF-Test had a high agreement k index (0,920), which was considered as Almost Perfect, in addition to differ from conventional techniques. Thus, focusing in the potential use of TF-Test for diagnosing different intestinal parasites, this work had been continued for developing a better prototype kit of TF-Test. In the second phase, six different specialized laboratories from public or private service had evaluated faecal samples collected from 1.244 patients. Data from those laboratories attested that TF-Test was actually better than other known techniques (Coprotest, Lutz and Lutz, Faust & Cols. and Rugai, Mattos & Brisola), for presenting sensibility range between 82,6% and 100%, which was significantly higher than conventional methods (p < 0,05). In four of the six laboratories, k index of TF-Test was classified as Almost Perfect, and in the other two laboratories, the index was considered as Substancial. The high sensibility of TF-Test in addition to its practice and economic aspects shown that TF-Test method is very appropriated for individual diagnosis, population inquires as well as for evaluating therapy programs in the control of intestinal parasitoses Ver menos
In order to obtain... Ver mais Abstract: Human intestinal parasitoses constitute a serious public health problem either in developing or developed countries. Those enteroparasitoses could be attributed to the increase of travelers around endemic areas and mainly due to immunocompromised patients.
In order to obtain sensible results and to have a high chance to find organisms by multiple sample exams, it is necessary at least three serial parasitologycal faecal tests. For these reasons, a practice and non-expensive kit (TF-Test) was developed for being industrialized by a specialized company (Immunoassay Ind. Com. Ltda., Sao Paulo, Brazil). This kit makes possible to carry out three different faecal samples each other day. The three faecal samples are separately collected into tubes containing a preservative solution. Laboratory evaluation is done in just one step by using a pool of the three faecal samples. Mixture mass is passing by a double filter and then centrifuged for concentrating the mixture. Thus, the present study reports about evaluations of a prototype kit developed into two different phases: first phase was a six months stage inside developer laboratory and, the second one was a two years stage involving six different collaborative laboratories. In the first phase, parasitologycal exams using TF-Test was carried out in 30 patients with intestinal parasites and in 30 patients free of intestinal parasites and, it was significantly more sensible (96,6%, p<0,05) compared with conventional techniques as Coprotest, StillQuick e Parasep or with those frequently used as Lutz or Hoffman, Pons & Janer. Furthermore, TF-Test had a high agreement k index (0,920), which was considered as Almost Perfect, in addition to differ from conventional techniques. Thus, focusing in the potential use of TF-Test for diagnosing different intestinal parasites, this work had been continued for developing a better prototype kit of TF-Test. In the second phase, six different specialized laboratories from public or private service had evaluated faecal samples collected from 1.244 patients. Data from those laboratories attested that TF-Test was actually better than other known techniques (Coprotest, Lutz and Lutz, Faust & Cols. and Rugai, Mattos & Brisola), for presenting sensibility range between 82,6% and 100%, which was significantly higher than conventional methods (p < 0,05). In four of the six laboratories, k index of TF-Test was classified as Almost Perfect, and in the other two laboratories, the index was considered as Substancial. The high sensibility of TF-Test in addition to its practice and economic aspects shown that TF-Test method is very appropriated for individual diagnosis, population inquires as well as for evaluating therapy programs in the control of intestinal parasitoses Ver menos
Dias, Luiz Cândido de Souza, 1943-
Orientador
Shimizu, Sumie Hoshino
Coorientador
Magalhães, Luiz Augusto, 1932-2017
Avaliador
Kawazoe, Urara, 1946-
Avaliador
Kanamura, Herminia Yohko
Avaliador
Avaliação de um novo kit (TF-TEST) nacional destinado ao diagnostico de enteroparasitoses em amostras fecais
Jancarlo Ferreira Gomes
Avaliação de um novo kit (TF-TEST) nacional destinado ao diagnostico de enteroparasitoses em amostras fecais
Jancarlo Ferreira Gomes
Exemplares
Nº de exemplares: 1
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