Proteomic analyses of koi herpesvirus by mass spectrometry reveal 40 structural proteins

[en] Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.

Disciplines :

Biochemistry, biophysics & molecular biology
Biotechnology

Author, co-author :

Michel, B.

Leroy, Baptiste ; Université de Mons > Faculté des Sciences > Protéomie et Microbiologie

Stalin Raj, V.

Lieffrig, F.

Mast, J.

Wattiez, Ruddy ; Université de Mons > Faculté des Sciences > Protéomie et Microbiologie

Vanderplasschen, A.

Costes, B.

Language :

English

Title :

Proteomic analyses of koi herpesvirus by mass spectrometry reveal 40 structural proteins

Publication date :

21 October 2010

Journal title :

Journal of General Virology

ISSN :

0022-1317

Publisher :

Society for General Microbiology, United Kingdom

Volume :

Issue :

Pages :

01-14

Peer reviewed :

Peer Reviewed verified by ORBi

Research unit :

S828 - Protéomie et Microbiologie

Available on ORBi UMONS :

since 10 June 2010

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