Magnetic Resonance Molecular Imaging of Vascular Cell Adhesion Molecule-1 Expression in Inflammatory Lesions Using a Peptide-Vectorized Paramagnetic Imaging Probe
Burtea, Carmen; Laurent, Sophie; Port, M.et al.
2009 • In Journal of Medicinal Chemistry, 52 (15), p. 4725-4742
[en] The vascular cell adhesion molecule-1 (VCAM-1) has distinct roles in inflammatory cell recruitment to the damaged vessel wall. In the present work, a cyclic heptapeptide phage displayed library was screened in vitro during four rounds of biopanning. On the basis of K-d and IC50 values, a peptide (encoded as R832) was selected for in vitro and in vivo validation. After conjugation to Gd-DOTA, VCAM-I imaging was assessed by MRI on a model of T cell mediated hepatitis, induced in mice by concanavalin A. On histological samples, the location of biotinylated R832 (R832-Bt) around liver veins in hepatitis resembles the pattern of M RI enhancement. Gd-DOTA-R832 was then assessed oil ApoE(-/-) mice and produced ail important signal enhancement of the aortic wall, while R832-Bt interacted with morphologic structures comparable to those marked by anti-VCAM-1 antibody. In conclusion, the in vitro and in vivo evaluation of peptide R832 suggests a specific interaction with the targeted biomolecule. Its conjugation to imaging reporters could assist the diagnosis of inflammatory diseases.
Research center :
CMMI - Centre de Recherche en Microscopie et Imagerie Médicale
Disciplines :
Cardiovascular & respiratory systems Immunology & infectious disease Radiology, nuclear medicine & imaging Biotechnology Chemistry
Author, co-author :
Burtea, Carmen ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie générale, organique et biomédicale
Laurent, Sophie ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie générale, organique et biomédicale
Port, M.
Lancelot, E.
Ballet, S.
Rousseaux, O.
Toubeau, Gérard
Vander Elst, Luce ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie générale, organique et biomédicale
Corot, C.
Muller, Robert ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie générale, organique et biomédicale
Language :
English
Title :
Magnetic Resonance Molecular Imaging of Vascular Cell Adhesion Molecule-1 Expression in Inflammatory Lesions Using a Peptide-Vectorized Paramagnetic Imaging Probe
Publication date :
01 January 2009
Journal title :
Journal of Medicinal Chemistry
ISSN :
0022-2623
Publisher :
American Chemical Society, United States - District of Columbia
Volume :
52
Issue :
15
Pages :
4725-4742
Peer reviewed :
Peer Reviewed verified by ORBi
Research unit :
M108 - Chimie générale, organique et biomédicale M118 - Histologie
Research institute :
R550 - Institut des Sciences et Technologies de la Santé R100 - Institut des Biosciences
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