Article (Scientific journals)
Targeted Analysis of HSP70 Isoforms in Human Spermatozoa in the Context of Capacitation and Motility
Grassi, Sarah; Bisconti, Marie; Martinet, Baptiste et al.
2022In International Journal of Molecular Sciences, 23 (12), p. 6497
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Keywords :
Molecular Biology; Spermatozoa; HSP70; Capacitation; Motility; Mass spectrometry
Abstract :
[en] HSP70s constitute a family of chaperones, some isoforms of which appear to play a role in sperm function. Notably, global proteomic studies analyzing proteins deregulated in asthenozoospermia, a main cause of male infertility characterized by low sperm motility, showed the dysregulation of some HSP70 isoforms. However, to date, no clear trend has been established since the variations in the abundance of HSP70 isoforms differed between studies. The HSPA2 isoform has been reported to play a key role in fertilization, but its dysregulation and possible relocation during capacitation, a maturation process making the spermatozoon capable of fertilizing an oocyte, is debated in the literature. The aim of the present study was to investigate the fate of all sperm HSP70 isoforms during capacitation and in relation to sperm motility. Using Multiple-Reaction Monitoring (MRM) mass spectrometry, we showed that the relative abundance of all detected isoforms was stable between non-capacitated and capacitated spermatozoa. Immunofluorescence using two different antibodies also demonstrated the stability of HSP70 isoform localization during capacitation. We also investigated spermatozoa purified from 20 sperm samples displaying various levels of total and progressive sperm motility. We showed that the abundance of HSP70 isoforms is not correlated to sperm total or progressive motility.
Disciplines :
Biochemistry, biophysics & molecular biology
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Grassi, Sarah
Bisconti, Marie ;  Université de Mons - UMONS > Faculté des Sciences > Service de Biologie cellulaire
Martinet, Baptiste 
Arcolia, Vanessa
Simon, Jean-François
Wattiez, Ruddy  ;  Université de Mons - UMONS > Faculté des Sciences > Service de Protéomie et Microbiologie
Leroy, Baptiste  ;  Université de Mons - UMONS > Faculté des Sciences > Service de Protéomie et Microbiologie
Hennebert, Elise  ;  Université de Mons - UMONS > Faculté des Sciences > Service de Biologie cellulaire
Language :
English
Title :
Targeted Analysis of HSP70 Isoforms in Human Spermatozoa in the Context of Capacitation and Motility
Publication date :
10 June 2022
Journal title :
International Journal of Molecular Sciences
ISSN :
1661-6596
eISSN :
1422-0067
Publisher :
MDPI AG
Volume :
23
Issue :
12
Pages :
6497
Peer reviewed :
Peer Reviewed verified by ORBi
Research institute :
R550 - Institut des Sciences et Technologies de la Santé
R100 - Institut des Biosciences
Funders :
Fund for Medical Research in Hainaut
Walloon Government
European Regional Development Fund
Fund for Scientific Research
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since 20 June 2022

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